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Literature summary for 2.5.1.31 extracted from

  • Chang, S.Y.; Chen, Y.K.; Wang, A.H.; Liang, P.H.
    Identification of the active conformation and the importance of length of the flexible loop 72-83 in regulating the conformational change of undecaprenyl pyrophosphate synthase (2003), Biochemistry, 42, 14452-14459.
    View publication on PubMed
Search for more literature for 2.5.1.31

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
using the hanging drop setup Escherichia coli

Protein Variants

Protein Variants Comment Organism
DELTAS72 loop-shortening mutant. Change of the length of the loop 72-83 impairs the ability of conformational change and causes remarkably lower activity of UPPs Escherichia coli
DELTAS83 loop-shortening mutant. Change of the length of the loop 72-83 impairs the ability of conformational change and causes remarkably lower activity of UPPs Escherichia coli
DELTAV82S83 loop-shortening mutant. Change of the length of the loop 72-83 impairs the ability of conformational change and causes remarkably lower activity of UPPs Escherichia coli
additional information loop-shortening mutants with deletion of V82 and S83 or S72 also adopt an open conformation with the loop stretched, although they show decreased intrinsic fluorescence with (2E,6E)-farnesyl diphosphate bound, similar to that seen in the wild-type enzyme Escherichia coli
S83(Ala)1 mutant with amino acids inserted into the loop Escherichia coli
S83(Ala)5 mutant with amino acids inserted into the loop Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00017
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C, S83(Ala)5 mutant Escherichia coli
0.0004
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C, wild type Escherichia coli
0.0004
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C, S83(Ala)1 mutant Escherichia coli
0.0005
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C, DELTAS72 mutant Escherichia coli
0.001
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C, DELTAS83 mutant Escherichia coli
0.0018
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C, DELTAV82S83 mutant Escherichia coli
0.0041
-
 isopentenyl diphosphate pH 7.5, 25°C, wild type Escherichia coli
0.0078
-
 isopentenyl diphosphate pH 7.5, 25°C, S83(Ala)5 mutant Escherichia coli
0.0117
-
 isopentenyl diphosphate pH 7.5, 25°C, DELTAS72 mutant Escherichia coli
0.034
-
 isopentenyl diphosphate pH 7.5, 25°C, S83(Ala)1 mutant Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Triton 3 Triton (T1, T2, and T3) are located in the two monomers with one on the top portion of the active site tunnel in monomer A and the other two occupying the overall tunnel of monomer B Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Escherichia coli 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer the two subunits show similar structures Escherichia coli

Synonyms

Synonyms Comment Organism
undecaprenyl pyrophosphate synthase
-
 Escherichia coli
UPPs
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00013
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C, S83(Ala)5 mutant Escherichia coli
0.00022
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C, DELTAV82S83 mutant Escherichia coli
0.00028
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C, DELTAS72 mutant Escherichia coli
0.00076
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C, DELTAS83 mutant Escherichia coli
0.0009
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C, S83(Ala)1 mutant Escherichia coli
2.5
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C, wild type Escherichia coli