Cloned (Comment) | Organism |
---|---|
expression of mutant enzmyes W31F, W75F, W91F, W149F, W207F, and W221F as His-tag fusion proteins using Escherichia coli BL21 as host cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
W149F | turnover-number is 68% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 1.75fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 4.9fold higher than that of the wild-type enzyme | Escherichia coli |
W207F | turnover-number is 60% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 8.5fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 5.6fold higher than that of the wild-type enzyme | Escherichia coli |
W221F | turnover-number is 140% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 1.3fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 3.4fold higher than that of the wild-type enzyme | Escherichia coli |
W31F | turnover-number is 44% of that of the wild-type enzyme,Km-value for farnesyl diphosphate is 5fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 1.4fold higher than that of the wild-type enzyme | Escherichia coli |
W75F | turnover-number is 44% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 4.5fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 6.3fold higher than that of the wild-type enzyme | Escherichia coli |
W91F | turnover-number is equal to that of the wild-type enzyme, Km-value for farnesyl diphosphate is equal to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 1.8fold higher than that of the wild-type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2E,6E)-farnesyl thiodiphosphate | (2E,6E)-farnesyl diphosphate analogue | Escherichia coli | |
S-farnesyl thiodiphosphate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
farnesyl diphosphate | wild-type enzyme, pH 7.5, 25°C | Escherichia coli | |
0.0004 | - |
farnesyl diphosphate | mutant enzyme W91F, pH 7.5, 25°C | Escherichia coli | |
0.0004 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W91F, pH 7.5, 25°C, wild-type enzyme, pH 7.5, 25°C | Escherichia coli | |
0.0005 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W221F, pH 7.5, 25°C | Escherichia coli | |
0.0005 | - |
farnesyl diphosphate | mutant enzyme W221F, pH 7.5, 25°C | Escherichia coli | |
0.0007 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W149F, pH 7.5, 25°C | Escherichia coli | |
0.0007 | - |
farnesyl diphosphate | mutant enzyme W149F, pH 7.5, 25°C | Escherichia coli | |
0.0018 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W75F, pH 7.5, 25°C | Escherichia coli | |
0.0018 | - |
farnesyl diphosphate | mutant enzyme W75F, pH 7.5, 25°C | Escherichia coli | |
0.002 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W31F, pH 7.5, 25°C | Escherichia coli | |
0.002 | - |
farnesyl diphosphate | mutant enzyme W31F, pH 7.5, 25°C | Escherichia coli | |
0.0034 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W207F, pH 7.5, 25°C | Escherichia coli | |
0.0034 | - |
farnesyl diphosphate | mutant enzyme W207F, pH 7.5, 25°C | Escherichia coli | |
0.0041 | - |
isopentenyl diphosphate | wild-type enzyme, pH 7.5, 25°C | Escherichia coli | |
0.0059 | - |
isopentenyl diphosphate | mutant enzyme W31F, pH 7.5, 25°C | Escherichia coli | |
0.0072 | - |
isopentenyl diphosphate | mutant enzyme W91F, pH 7.5, 25°C | Escherichia coli | |
0.014 | - |
isopentenyl diphosphate | mutant enzyme W221F, pH 7.5, 25°C | Escherichia coli | |
0.02 | - |
isopentenyl diphosphate | mutant enzyme W149F, pH 7.5, 25°C | Escherichia coli | |
0.023 | - |
isopentenyl diphosphate | mutant enzyme W207F, pH 7.5, 25°C | Escherichia coli | |
0.026 | - |
isopentenyl diphosphate | mutant enzyme W75F, pH 7.5, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Escherichia coli | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl thiodiphosphate + isopentenyl diphosphate | weak activity, 10000000fold lower than with (2E,6E)-farnesyl diphosphate | Escherichia coli | ? | - |
? | |
isopentenyl diphosphate + farnesyl diphosphate | - |
Escherichia coli | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
isopentenyl diphosphate + S-farnesyl thiodiphosphate | weak activity, 10000000fold lower than with farnesyl diphosphate | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
undecaprenyl pyrophosphate synthase | - |
Escherichia coli |
UPPs | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
0.00000031 | - |
(2E,6E)-farnesyl thiodiphosphate | much smaller than using (2E,6E)-farnesyl diphosphate, pH 7.5, 25°C | Escherichia coli | |
1.1 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W31F, pH 7.5, 25°C | Escherichia coli | |
1.1 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W75F, pH 7.5, 25°C | Escherichia coli | |
1.5 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W207F, pH 7.5, 25°C | Escherichia coli | |
1.7 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W149F, pH 7.5, 25°C | Escherichia coli | |
2.5 | - |
(2E,6E)-farnesyl diphosphate | wild-type enzyme, pH 7.5, 25°C | Escherichia coli | |
2.5 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W91F, pH 7.5, 25°C | Escherichia coli | |
3.5 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W221F, pH 7.5, 25°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
(S)-farnesyl thiopyrophosphate | - |
Escherichia coli | |
0.0002 | - |
(2E,6E)-farnesyl thiodiphosphate | pH 7.5, 25°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
347 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W91F, pH 7.5, 25°C | Escherichia coli | |
441 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W207F, pH 7.5, 25°C | Escherichia coli | |
550 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W31F, pH 7.5, 25°C | Escherichia coli | |
611 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W75F, pH 7.5, 25°C | Escherichia coli | |
2428 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W149F, pH 7.5, 25°C | Escherichia coli | |
6250 | - |
(2E,6E)-farnesyl diphosphate | wild-type enzyme, pH 7.5, 25°C | Escherichia coli | |
7000 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W221F, pH 7.5, 25°C | Escherichia coli |