Cloned (Comment) | Organism |
---|---|
mutated enzymes are overproduced in Escherichia coli | Micrococcus luteus |
Protein Variants | Comment | Organism |
---|---|---|
D29A | Km-value for farnesyl diphosphate is 9.2fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 5.8fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 1% of that of the wild-type enzyme | Micrococcus luteus |
D29A | Km-value for farnesyl diphosphate is 9.2fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 5.8fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 1% of that of the wild-type enzyme | Micrococcus luteus |
E76Q | Km-value for farnesyl diphosphate is 1.6fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 3.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.5fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 30% of that of the wild-type enzyme | Micrococcus luteus |
E76Q | Km-value for farnesyl diphosphate is 1.6fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 3.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.5fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 30% of that of the wild-type enzyme | Micrococcus luteus |
E84Q | Km-value for farnesyl diphosphate is 3.2fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.98fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 65% of that of the wild-type enzyme | Micrococcus luteus |
E84Q | Km-value for farnesyl diphosphate is 3.2fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.98fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 65% of that of the wild-type enzyme | Micrococcus luteus |
G32R | Km-value for farnesyl diphosphate is comparable to that of the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.9fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is comparable to that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 27% of that of the wild-type enzyme | Micrococcus luteus |
G32R | Km-value for farnesyl diphosphate is comparable to that of the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.9fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is comparable to that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 27% of that of the wild-type enzyme | Micrococcus luteus |
G32R/R42G | Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.4fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 58% of that of the wild-type enzyme | Micrococcus luteus |
G32R/R42G | Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.4fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 58% of that of the wild-type enzyme | Micrococcus luteus |
R33A | Km-value for farnesyl diphosphate is 40fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.2fold lower than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 3% of that of the wild-type enzyme. Different product distribution pattern compared to the wildf-type enzyme | Micrococcus luteus |
R33A | Km-value for farnesyl diphosphate is 40fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.2fold lower than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 3% of that of the wild-type enzyme. Different product distribution pattern compared to the wildf-type enzyme | Micrococcus luteus |
R42G | Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.8fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.1fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 13% of that of the wild-type enzyme | Micrococcus luteus |
R42G | Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.8fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.1fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 13% of that of the wild-type enzyme | Micrococcus luteus |
R80A | Km-value for farnesyl diphosphate is 6.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 6.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is less than 1% of that of the wild-type enzyme | Micrococcus luteus |
R80A | Km-value for farnesyl diphosphate is 6.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 6.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is less than 1% of that of the wild-type enzyme | Micrococcus luteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SO42- | competitive inhibitor with respect to (2E,6E)-farnesyl diphosphate; competitive inhibitor with respect to farnesyl diphosphate | Micrococcus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
farnesyl diphosphate | mutant enzyme E76Q, pH 7.5, 37°C | Micrococcus luteus | |
0.0023 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme E76Q, pH 7.5, 37°C | Micrococcus luteus | |
0.0023 | - |
(2E,6E)-farnesyl diphosphate | E76Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0033 | - |
farnesyl diphosphate | mutant enzyme G32R, pH 7.5, 37°C | Micrococcus luteus | |
0.0033 | - |
(2E,6E)-farnesyl diphosphate | G32R mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0036 | - |
farnesyl diphosphate | wild-type enzyme, pH 7.5, 37°C | Micrococcus luteus | |
0.0036 | - |
(2E,6E)-farnesyl diphosphate | wild-type, pH 7.5, 37°C | Micrococcus luteus | |
0.0043 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme G32R, pH 7.5, 37°C | Micrococcus luteus | |
0.0043 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | G32R mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0045 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme R42G, pH 7.5, 37°C | Micrococcus luteus | |
0.0045 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0049 | - |
farnesyl diphosphate | mutant enzyme R42G, pH 7.5, 37°C | Micrococcus luteus | |
0.0049 | - |
(2E,6E)-farnesyl diphosphate | R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.005 | - |
farnesyl diphosphate | mutant enzyme G32R/R42G, pH 7.5, 37°C | Micrococcus luteus | |
0.005 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme D29A, pH 7.5, 37°C | Micrococcus luteus | |
0.005 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | D29A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.005 | - |
(2E,6E)-farnesyl diphosphate | G32R/R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0059 | - |
isopentenyl diphosphate | mutant enzyme G32R/R42G, pH 7.5, 37°C | Micrococcus luteus | |
0.0059 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme G32R/R42G, pH 7.5, 37°C | Micrococcus luteus | |
0.0059 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | G32R-R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0059 | - |
isopentenyl diphosphate | G32R/R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.006 | - |
isopentenyl diphosphate | mutant enzyme G32R, pH 7.5, 37°C | Micrococcus luteus | |
0.006 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme E84Q, pH 7.5, 37°C | Micrococcus luteus | |
0.006 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | E84Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.006 | - |
isopentenyl diphosphate | G32R mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0061 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme R80A, pH 7.5, 37°C | Micrococcus luteus | |
0.0061 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | R80A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0078 | - |
isopentenyl diphosphate | wild-type enzyme, pH 7.5, 37°C | Micrococcus luteus | |
0.0078 | - |
isopentenyl diphosphate | wild-type, pH 7.5, 37°C | Micrococcus luteus | |
0.0082 | - |
(Z,E,E)-Geranylgeranyl diphosphate | wild-type enzyme, pH 7.5, 37°C | Micrococcus luteus | |
0.0082 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | wild-type, pH 7.5, 37°C | Micrococcus luteus | |
0.0088 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | wild-type, pH 7.5, 37°C | Micrococcus luteus | |
0.0095 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme R33A, pH 7.5, 37°C | Micrococcus luteus | |
0.0095 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | R33A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0114 | - |
isopentenyl diphosphate | mutant enzyme E76Q, pH 7.5, 37°C | Micrococcus luteus | |
0.0114 | - |
farnesyl diphosphate | mutant enzyme E84Q, pH 7.5, 37°C | Micrococcus luteus | |
0.0114 | - |
isopentenyl diphosphate | E76Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0114 | - |
(2E,6E)-farnesyl diphosphate | E84Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.023 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | E76Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0231 | - |
farnesyl diphosphate | mutant enzyme R80A, pH 7.5, 37°C | Micrococcus luteus | |
0.0231 | - |
(2E,6E)-farnesyl diphosphate | R80A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0242 | - |
isopentenyl diphosphate | mutant enzyme R42G, pH 7.5, 37°C | Micrococcus luteus | |
0.0242 | - |
isopentenyl diphosphate | R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0307 | - |
isopentenyl diphosphate | mutant enzyme E84Q, pH 7.5, 37°C | Micrococcus luteus | |
0.0307 | - |
isopentenyl diphosphate | E84Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0332 | - |
farnesyl diphosphate | mutant enzyme D29A, pH 7.5, 37°C | Micrococcus luteus | |
0.0332 | - |
(2E,6E)-farnesyl diphosphate | D29A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0452 | - |
isopentenyl diphosphate | mutant enzyme D29A, pH 7.5, 37°C | Micrococcus luteus | |
0.0452 | - |
isopentenyl diphosphate | D29A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0491 | - |
isopentenyl diphosphate | mutant enzyme R80A, pH 7.5, 37°C | Micrococcus luteus | |
0.0491 | - |
isopentenyl diphosphate | R80A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.064 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | R33A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.144 | - |
farnesyl diphosphate | mutant enzyme R33A, pH 7.5, 37°C | Micrococcus luteus | |
0.144 | - |
(2E,6E)-farnesyl diphosphate | R33A mutant, pH 7.5, 37°C | Micrococcus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Micrococcus luteus | catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate | ? | - |
? | |
additional information | Micrococcus luteus B-P 26 | catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | - |
B-P 26 | - |
Micrococcus luteus | O82827 | B-P 26 | - |
Micrococcus luteus B-P 26 | - |
B-P 26 | - |
Micrococcus luteus B-P 26 | O82827 | B-P 26 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Micrococcus luteus | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Micrococcus luteus B-P 26 | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Micrococcus luteus | ? | - |
? | |
(2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Micrococcus luteus B-P 26 | ? | - |
? | |
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Micrococcus luteus | ? | - |
? | |
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Micrococcus luteus B-P 26 | ? | - |
? | |
isopentenyl diphosphate + farnesyl diphosphate | (E,E)-farnesyl diphosphate | Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
isopentenyl diphosphate + farnesyl diphosphate | (E,E)-farnesyl diphosphate | Micrococcus luteus B-P 26 | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
isopentenyl diphosphate + geranylgeranyl diphosphate | (Z,E,E)-geranylgeranyl diphosphate | Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
isopentenyl diphosphate + geranylgeranyl diphosphate | (Z,E,E)-geranylgeranyl diphosphate | Micrococcus luteus B-P 26 | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
additional information | catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate | Micrococcus luteus | ? | - |
? | |
additional information | catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate | Micrococcus luteus B-P 26 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
undecaprenyl diphosphate synthase | - |
Micrococcus luteus |
UPP synthase | - |
Micrococcus luteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Micrococcus luteus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
(2E,6E)-farnesyl diphosphate | R80A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.00579 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | R80A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0167 | - |
farnesyl diphosphate | mutant enzyme D29A, pH 7.5, 37°C | Micrococcus luteus | |
0.0167 | - |
(2E,6E)-farnesyl diphosphate | D29A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0258 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | D29A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0542 | - |
farnesyl diphosphate | mutant enzyme R33A, pH 7.5, 37°C | Micrococcus luteus | |
0.0542 | - |
(2E,6E)-farnesyl diphosphate | R33A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.0813 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | R33A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.216 | - |
farnesyl diphosphate | mutant enzyme R42G, pH 7.5, 37°C | Micrococcus luteus | |
0.216 | - |
(2E,6E)-farnesyl diphosphate | R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.382 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.462 | - |
farnesyl diphosphate | mutant enzyme G32R, pH 7.5, 37°C | Micrococcus luteus | |
0.462 | - |
(2E,6E)-farnesyl diphosphate | G32R mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.505 | - |
farnesyl diphosphate | mutant enzyme E76Q, pH 7.5, 37°C | Micrococcus luteus | |
0.505 | - |
(2E,6E)-farnesyl diphosphate | E76Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.604 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | E76Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.608 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | G32R mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.96 | - |
farnesyl diphosphate | mutant enzyme G32R/R42G, pH 7.5, 37°C | Micrococcus luteus | |
0.96 | - |
(2E,6E)-farnesyl diphosphate | G32R/R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
1.07 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | E84Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
1.09 | - |
farnesyl diphosphate | mutant enzyme E84Q, pH 7.5, 37°C | Micrococcus luteus | |
1.09 | - |
(2E,6E)-farnesyl diphosphate | E84Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
1.11 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | G32R/R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
1.66 | - |
farnesyl diphosphate | wild-type enzyme, pH 7.5, 37°C | Micrococcus luteus | |
1.66 | - |
(2E,6E)-farnesyl diphosphate | wild-type, pH 7.5, 37°C | Micrococcus luteus | |
1.77 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | wild-type, pH 7.5, 37°C | Micrococcus luteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Micrococcus luteus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
SO42- | - |
Micrococcus luteus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
(2E,6E)-farnesyl diphosphate | R80A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.713 | - |
(2E,6E)-farnesyl diphosphate | R33A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.777 | - |
(2E,6E)-farnesyl diphosphate | D29A mutant, pH 7.5, 37°C | Micrococcus luteus | |
0.949 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | R80A mutant, pH 7.5, 37°C | Micrococcus luteus | |
5.2 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | D29A mutant, pH 7.5, 37°C | Micrococcus luteus | |
8.65 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | R33A mutant, pH 7.5, 37°C | Micrococcus luteus | |
26.3 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | E76Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
77.9 | - |
(2E,6E)-farnesyl diphosphate | R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
84.8 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
93.9 | - |
(2E,6E)-farnesyl diphosphate | E84Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
141 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | G32R mutant, pH 7.5, 37°C | Micrococcus luteus | |
178 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | E84Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
184 | - |
(2E,6E)-farnesyl diphosphate | G32R mutant, pH 7.5, 37°C | Micrococcus luteus | |
188 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | G32R/R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
215.9 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | wild-type, pH 7.5, 37°C | Micrococcus luteus | |
222 | - |
(2E,6E)-farnesyl diphosphate | G32R/R42G mutant, pH 7.5, 37°C | Micrococcus luteus | |
262 | - |
(2E,6E)-farnesyl diphosphate | E76Q mutant, pH 7.5, 37°C | Micrococcus luteus | |
461 | - |
(2E,6E)-farnesyl diphosphate | wild-type, pH 7.5, 37°C | Micrococcus luteus |