Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Micrococcus luteus |
Protein Variants | Comment | Organism |
---|---|---|
N77A | dramatic decrease in catalytic activity, but Km-values for both allylic and homoallylic substrates are comparable to those of the wild-type | Micrococcus luteus |
N77D | dramatic decrease in catalytic activity, but Km-values for both allylic and homoallylic substrates are comparable to those of the wild-type | Micrococcus luteus |
N77Q | dramatic decrease in catalytic activity, but Km-values for both allylic and homoallylic substrates are comparable to those of the wild-type | Micrococcus luteus |
W78D | moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 18.7fold increase in Km-value for farnesyl diphosphate | Micrococcus luteus |
W78I | moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 6-fold increase in Km-value for farnesyl diphosphate, 2.3fold increase in Km-value for (Z,E,E)-geranylgeranyl diphosphate | Micrococcus luteus |
W78I | moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 6-fold increase in Km-value for farnesyl diphosphate, 2.3fold increase in Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate | Micrococcus luteus |
W78R | moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 14.5-fold increase in Km-value for farnesyl diphosphate, 1.8fold increase in Km-value for (Z,E,E)-geranylgeranyl diphosphate | Micrococcus luteus |
W78R | moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 14.5-fold increase in Km-value for farnesyl diphosphate, 1.8fold increase in Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate | Micrococcus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | mutant enzyme N77Q, pH 7.5, 37°C | Micrococcus luteus | |
0.005 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme N77Q, pH 7.5, 37°C | Micrococcus luteus | |
0.0078 | - |
isopentenyl diphosphate | wild-type enzyme, pH 7.5, 37°C | Micrococcus luteus | |
0.008 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme N77D, pH 7.5, 37°C | Micrococcus luteus | |
0.008 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme W78D, pH 7.5, 37°C | Micrococcus luteus | |
0.008 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | mutant enzyme N77D, pH 7.5, 37°C; mutant enzyme W78D, pH 7.5, 37°C | Micrococcus luteus | |
0.0082 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | wild-type enzyme, pH 7.5, 37°C | Micrococcus luteus | |
0.0082 | - |
(Z,E,E)-Geranylgeranyl diphosphate | wild-type enzyme, pH 7.5, 37°C | Micrococcus luteus | |
0.0083 | - |
(2E,6E)-farnesyl diphosphate | wild-type, pH 7.5, 37°C | Micrococcus luteus | |
0.0083 | - |
farnesyl diphosphate | wild-type, pH 7.5, 37°C | Micrococcus luteus | |
0.009 | - |
isopentenyl diphosphate | mutant enzyme N77Q, pH 7.5, 37°C | Micrococcus luteus | |
0.0102 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | mutant enzyme N77A, pH 7.5, 37°C | Micrococcus luteus | |
0.0102 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme N77A, pH 7.5, 37°C | Micrococcus luteus | |
0.0104 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N77Q, pH 7.5, 37°C | Micrococcus luteus | |
0.0104 | - |
farnesyl diphosphate | mutant enzyme N77Q, pH 7.5, 37°C | Micrococcus luteus | |
0.012 | - |
isopentenyl diphosphate | mutant enzyme N77A, pH 7.5, 37°C | Micrococcus luteus | |
0.0131 | - |
isopentenyl diphosphate | mutant enzyme W78D, pH 7.5, 37°C | Micrococcus luteus | |
0.0139 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N77D, pH 7.5, 37°C | Micrococcus luteus | |
0.0139 | - |
farnesyl diphosphate | mutant enzyme N77D, pH 7.5, 37°C | Micrococcus luteus | |
0.0141 | - |
isopentenyl diphosphate | mutant enzyme W78I, pH 7.5, 37°C | Micrococcus luteus | |
0.0147 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N77A, pH 7.5, 37°C | Micrococcus luteus | |
0.0147 | - |
farnesyl diphosphate | mutant enzyme N77A, pH 7.5, 37°C | Micrococcus luteus | |
0.015 | - |
isopentenyl diphosphate | mutant enzyme N77D, pH 7.5, 37°C | Micrococcus luteus | |
0.015 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | mutant enzyme W78R, pH 7.5, 37°C | Micrococcus luteus | |
0.015 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme W78R, pH 7.5, 37°C | Micrococcus luteus | |
0.0165 | - |
isopentenyl diphosphate | mutant enzyme W78R, pH 7.5, 37°C | Micrococcus luteus | |
0.019 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | mutant enzyme W78I, pH 7.5, 37°C | Micrococcus luteus | |
0.019 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme W78I, pH 7.5, 37°C | Micrococcus luteus | |
0.0495 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W78I, pH 7.5, 37°C | Micrococcus luteus | |
0.0495 | - |
farnesyl diphosphate | mutant enzyme W78I, pH 7.5, 37°C | Micrococcus luteus | |
0.1207 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W78R, pH 7.5, 37°C | Micrococcus luteus | |
0.1207 | - |
farnesyl diphosphate | mutant enzyme W78R, pH 7.5, 37°C | Micrococcus luteus | |
0.155 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N78D, pH 7.5, 37°C | Micrococcus luteus | |
0.155 | - |
farnesyl diphosphate | mutant enzyme N78D, pH 7.5, 37°C | Micrococcus luteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | O82827 | B-P 26 | - |
Micrococcus luteus B-P 26 | O82827 | B-P 26 | - |
Purification (Comment) | Organism |
---|---|
- |
Micrococcus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Micrococcus luteus | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Micrococcus luteus B-P 26 | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Micrococcus luteus | ? | - |
? | |
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Micrococcus luteus B-P 26 | ? | - |
? | |
isopentenyl diphosphate + farnesyl diphosphate | - |
Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
isopentenyl diphosphate + farnesyl diphosphate | - |
Micrococcus luteus B-P 26 | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
isopentenyl diphosphate + geranylgeranyl diphosphate | - |
Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
isopentenyl diphosphate + geranylgeranyl diphosphate | - |
Micrococcus luteus B-P 26 | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
undecaprenyl diphosphate synthase | - |
Micrococcus luteus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00125 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N77A, pH 7.5, 37°C | Micrococcus luteus | |
0.00125 | - |
farnesyl diphosphate | mutant enzyme N77A, pH 7.5, 37°C | Micrococcus luteus | |
0.00155 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N77Q, pH 7.5, 37°C | Micrococcus luteus | |
0.00155 | - |
farnesyl diphosphate | mutant enzyme N77Q, pH 7.5, 37°C | Micrococcus luteus | |
0.002 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | mutant enzyme N77A, pH 7.5, 37°C | Micrococcus luteus | |
0.002 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme N77A, pH 7.5, 37°C | Micrococcus luteus | |
0.00231 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | mutant enzyme N77Q, pH 7.5, 37°C | Micrococcus luteus | |
0.00231 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme N77Q, pH 7.5, 37°C | Micrococcus luteus | |
0.0027 | - |
isopentenyl diphosphate | mutant enzyme N77Q, pH 7.5, 37°C | Micrococcus luteus | |
0.00272 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N77D, pH 7.5, 37°C | Micrococcus luteus | |
0.00272 | - |
farnesyl diphosphate | mutant enzyme N77D, pH 7.5, 37°C | Micrococcus luteus | |
0.00415 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | mutant enzyme N77D, pH 7.5, 37°C | Micrococcus luteus | |
0.00415 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme N77D, pH 7.5, 37°C | Micrococcus luteus | |
0.00473 | - |
isopentenyl diphosphate | mutant enzyme N77D, pH 7.5, 37°C | Micrococcus luteus | |
0.0164 | - |
isopentenyl diphosphate | mutant enzyme N77A, pH 7.5, 37°C | Micrococcus luteus | |
0.151 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W78D, pH 7.5, 37°C | Micrococcus luteus | |
0.151 | - |
farnesyl diphosphate | mutant enzyme W78D, pH 7.5, 37°C | Micrococcus luteus | |
0.3 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | mutant enzyme W78D, pH 7.5, 37°C | Micrococcus luteus | |
0.3 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme W78D, pH 7.5, 37°C | Micrococcus luteus | |
0.397 | - |
isopentenyl diphosphate | mutant enzyme W78D, pH 7.5, 37°C | Micrococcus luteus | |
0.434 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N78R, pH 7.5, 37°C | Micrococcus luteus | |
0.434 | - |
farnesyl diphosphate | mutant enzyme N78R, pH 7.5, 37°C | Micrococcus luteus | |
0.456 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme W78I, pH 7.5, 37°C | Micrococcus luteus | |
0.456 | - |
farnesyl diphosphate | mutant enzyme W78I, pH 7.5, 37°C | Micrococcus luteus | |
0.783 | - |
isopentenyl diphosphate | mutant enzyme W78I, pH 7.5, 37°C | Micrococcus luteus | |
0.803 | - |
isopentenyl diphosphate | mutant enzyme W78R, pH 7.5, 37°C | Micrococcus luteus | |
0.899 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | mutant enzyme W78R, pH 7.5, 37°C | Micrococcus luteus | |
0.899 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme W78R, pH 7.5, 37°C | Micrococcus luteus | |
1.22 | - |
(2E,6E)-farnesyl diphosphate | wild-type enzyme, pH 7.5, 37°C | Micrococcus luteus | |
1.22 | - |
farnesyl diphosphate | wild-type enzyme, pH 7.5, 37°C | Micrococcus luteus | |
1.37 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | mutant enzyme W78I, pH 7.5, 37°C | Micrococcus luteus | |
1.37 | - |
(Z,E,E)-Geranylgeranyl diphosphate | mutant enzyme W78I, pH 7.5, 37°C | Micrococcus luteus | |
1.77 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | wild-type enzyme, pH 7.5, 37°C | Micrococcus luteus | |
1.77 | - |
(Z,E,E)-Geranylgeranyl diphosphate | wild-type enzyme, pH 7.5, 37°C | Micrococcus luteus | |
1.85 | - |
isopentenyl diphosphate | wild-type, pH 7.5, 37°C | Micrococcus luteus |