Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. Thioredoxin and His tag are utilized to increase the solubility of the recombinant protein and facilitate its purification using Ni-nitrilotriacetic acid column | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
isopentenyl diphosphate | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the recombinant enzyme expressed in Escherichia coli mostly exists in pellet in the absence of detergents, a low quantity of soluble enzyme is purified | Saccharomyces cerevisiae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | the synthesized long-chain dehydrodolichyl diphosphate serves as a precursor of glycosyl carrier in glycoprotein biosynthesis in eukaryotes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Renatured (Comment) | Organism |
---|---|
the recombinant protein in the pellet is solubilized with 7 M urea and purified using nickel-nitrilotriacetic acid under denaturing conditions. The protein refolding is achieved via the stepwise dialysis to remove the denaturant in the presence of 6 mM beta-mercaptoethanol. Alternatively, on-column refolding is carried out in a single step to obtain the active protein in large quantities. beta-Mercaptoethanol and Triton are both required in this quick refolding process | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate + farnesyl diphosphate | - |
Saccharomyces cerevisiae | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | the C55 polymer is the major product in presence of Triton X-100, without Triton C55-C75 polyprenyl diphosphates are generated | ? | |
additional information | the synthesized long-chain dehydrodolichyl diphosphate serves as a precursor of glycosyl carrier in glycoprotein biosynthesis in eukaryotes | Saccharomyces cerevisiae | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
0.0002 | - |
isopentenyl diphosphate | - |
Saccharomyces cerevisiae |