Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phospholipid | - |
Escherichia coli | |
Triton | 0.01 microM of UPPs with 5 microM of (2E,6E)-farnesyl diphosphate and 50 microM of isopentenyl diphosphate, 0.5 mM MgCl2, and 50 mM KCl in Hepes buffer (pH 7.5) is 190fold less active (0.013 s-1) for the IPP consumption in the absence of Triton | Escherichia coli | |
Triton | the UPPs steady-state kcat value in the presence of 0.1% Triton is 190fold larger than in the absence of Triton | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C | Escherichia coli | |
0.0004 | - |
(2E,6E)-farnesyl diphosphate | wild-type enzyme, pH 7.5, 25°C | Escherichia coli | |
0.0004 | - |
farnesyl diphosphate | wild-type enzyme, pH 7.5, 25°C | Escherichia coli | |
0.0041 | - |
isopentenyl diphosphate | pH 7.5, 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependend on | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | Escherichia coli | catalyzes the condensation of eight molecules of isopentenyl pyrophosphate (IPP) with farnesyl diphosphate (FPP) to generate C55 undecaprenyl diphosphate. With a variety of different ratios of enzyme to substrate and FPP to IPP in the presence or absence of Triton, different product distributions are found | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
His-tagged UPPs | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.013 | - |
pH 7.5, 25°C, absence of Triton | Escherichia coli |
2.5 | - |
pH 7.5, 25°C, in presence of 0.1% Triton | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Escherichia coli | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Escherichia coli | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | quantification of product distribution of UPPs catalyzed coupling of (2E,6E)-farnesyl diphosphate and isopentenyl diphosphate under various reaction conditions | ? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | catalyzes the condensation of eight molecules of isopentenyl pyrophosphate (IPP) with farnesyl diphosphate (FPP) to generate C55 undecaprenyl diphosphate. With a variety of different ratios of enzyme to substrate and FPP to IPP in the presence or absence of Triton, different product distributions are found | Escherichia coli | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
isopentenyl diphosphate + farnesyl diphosphate | - |
Escherichia coli | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | product distribution under various reaction conditions. In the presence of excess farnesyl diphosphate the intermediates C25-C50 accumulate | ? |
Synonyms | Comment | Organism |
---|---|---|
undecaprenyl pyrophosphate synthase | - |
Escherichia coli |
UPPs | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, without 0.1% Triton | Escherichia coli | |
2.5 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, in the presence of 0.1% Triton | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |