Literature summary for 2.5.1.3 extracted from

Saab-Rincon, G.; Olvera, L.; Olvera, M.; Rudino-Pinera, E.; Benites, E.; Soberon, X.; Morett, E.
Evolutionary walk between (beta/alpha)8 barrels: catalytic migration from triosephosphate isomerase to thiamin phosphate synthase (2012), J. Mol. Biol., 416, 255-270.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in DELTAthiE-Escherichia coli cells	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0007	-	2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate	wild type enzyme, in buffer containing 50 mM phosphate and 10 mM MgSO4, at pH 8.0 and 37°C	Escherichia coli
0.0012	-	4-methyl-5-beta-hydroxyethylthiazole phosphate	wild type enzyme, in buffer containing 50 mM phosphate and 10 mM MgSO4, at pH 8.0 and 37°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.7	-	wild type enzyme, in buffer containing 50 mM phosphate and 10 mM MgSO4, at pH 8.0 and 37°C	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-beta-hydroxyethylthiazole phosphate	-	Escherichia coli	thiamine phosphate + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
thiamin phosphate synthase	-	Escherichia coli
ThiE	-	Escherichia coli
TPS	-	Escherichia coli

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Release 2023.1 (January 2023)