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Literature summary for 2.5.1.3 extracted from

  • Reddick, J.J.; Nicewonger, R.; Begley, T.P.
    Mechanistic studies on thiamin phosphate synthase: evidence for a dissociative mechanism (2001), Biochemistry, 40, 10095-10102.
    View publication on PubMed
Search for more literature for 2.5.1.3

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
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-
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Purification (Commentary)

Purification (Comment) Organism
from E. coli overexpression strain Bacillus subtilis

Reaction

Reaction Comment Organism Reaction ID
4-amino-2-methyl-5-(diphosphomethyl)pyrimidine + 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate = diphosphate + thiamine phosphate + CO2 dissociative SN1 mechanism Bacillus subtilis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
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 Bacillus subtilis thiamine monophosphate + diphosphate
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 ?
4-amino-5-(hydroxymethyl)-2-(trifluoromethyl)pyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole poor substrate Bacillus subtilis ?
-
 ?
4-amino-5-(hydroxymethyl)-2-methoxypyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole good substrate Bacillus subtilis ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
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 additional information
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 Bacillus subtilis