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Literature summary for 2.5.1.3 extracted from
- Purification and properties of thiaminephosphate pyrophosphorylase of Escherichia coli (1973), Arch. Biochem. Biophys., 158, 242-248.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl phosphate | 0.5 mM, 50% inhibition, uncompetitive with respect to both substrates | Escherichia coli |  |
| ADP | - |
Escherichia coli | |
| ATP | uncompetitive with respect to both substrates | Escherichia coli | |
| CTP | - |
Escherichia coli | |
| diphosphate | - |
Escherichia coli | |
| GTP | - |
Escherichia coli | |
| phosphocreatine | - |
Escherichia coli | |
| phosphoenolpyruvate | - |
Escherichia coli | |
| UTP | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate | - |
Escherichia coli | |
| 0.00085 | - |
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates, 20% as effective as Mg2+ | Escherichia coli | |
| Co2+ | stimulates, 20% as effective as Mg2+ | Escherichia coli | |
| Mg2+ | required | Escherichia coli | |
| Mg2+ | Km: 0.063 mM | Escherichia coli | |
| Mg2+ | most effective at 6 mM | Escherichia coli | |
| Mn2+ | stimulates, at 6 mM equally effective as Mg2+ | Escherichia coli | |
| Zn2+ | stimulates, 20% as effective as Mg2+ | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.065 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole | - |
Escherichia coli | thiamine monophosphate + diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
5 min, without addition of both substrates, completely inactivated | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Escherichia coli |
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