Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate | pH 9.2, 37°C | Saccharomyces cerevisiae | |
0.007 | - |
2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate | pH 9.2, 37°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae | |
Mg2+ | 1 mM, 3-6fold stimulation | Saccharomyces cerevisiae | |
Mn2+ | half as effective as Mg2+ | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-15°C, 6 months, less than 10% loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole | - |
Saccharomyces cerevisiae | thiamine monophosphate + diphosphate | - |
? | |
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole | - |
Saccharomyces cerevisiae | thiamine monophosphate + diphosphate | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
21 | - |
room temperature, completely inactivated overnight | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.7 | 10 | about 40% of activity maximum at pH 7.7 and pH 10.0 | Saccharomyces cerevisiae |