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Literature summary for 2.5.1.29 extracted from

  • Chen, W.; He, S.; Liu, D.; Patil, G.B.; Zhai, H.; Wang, F.; Stephenson, T.J.; Wang, Y.; Wang, B.; Valliyodan, B.; Nguyen, H.T.; Liu, Q.
    A sweetpotato geranylgeranyl pyrophosphate synthase gene, IbGGPS, increases carotenoid content and enhances osmotic stress tolerance in Arabidopsis thaliana (2015), PLoS ONE, 10, e0137623.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene IbGGPS, sequence comparisons, transient expression of the GFP-tagged enzyme in specific areas of the plasma membrane of epidermal cells of onion Allium sp. and in chloroplasts of leaves of Nicotiana benthaminana. The coding region of IbGGPS is cloned into a binary vector under the control of 35S promoter and then transformed into Arabidopsis thaliana ecotype Columbia-0 to obtain transgenic plants. Transformations are made using Agrobacterium tumefaciens strain EHA105, semi- and quantitative real-time PCR expression analysis Ipomoea batatas

Protein Variants

Protein Variants Comment Organism
additional information expression of the sweetpotato geranylgeranyl pyrophosphate synthase gene IbGGPS in Arabidopsis thaliana increases carotenoid content and enhances osmotic stress tolerance in the transgenic plants. Transgenic seedlings grow significantly longer roots compared to wild-type. Quantitative RT-PCR analysis shows altered expressions of several genes involved in the carotenoid biosynthesis in transgenic plants Ipomoea batatas

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Ipomoea batatas 9507
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plasma membrane
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Ipomoea batatas 5886
-

Organism

Organism UniProt Comment Textmining
Ipomoea batatas G8XQT1 cv. Nongdafu 14, gene IbGGPS
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Source Tissue

Source Tissue Comment Organism Textmining
additional information analysis of enzyme expression tissue distribution by quantitative real-time PCR Ipomoea batatas
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storage root
-
Ipomoea batatas
-

Synonyms

Synonyms Comment Organism
geranylgeranyl pyrophosphate synthase
-
Ipomoea batatas
GGPS
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Ipomoea batatas
IbGGPS
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Ipomoea batatas

General Information

General Information Comment Organism
physiological function the formation of geranylgeranyl diphosphate is a key step in biosynthetic pathway of carotenoids and many other terpenes. This step is catalyzed by geranylgeranyl diphosphate synthase. Expression of enzyme-encoding gene IbGGPS is likely associated with carotenoid profiles in storage roots. Sweet potato geranylgeranyl pyrophosphate synthase gene IbGGPS increases carotenoid content and enhances osmotic stress tolerance in Arabidopsis thaliana Ipomoea batatas