Cloned (Comment) | Organism |
---|---|
gene IbGGPS, sequence comparisons, transient expression of the GFP-tagged enzyme in specific areas of the plasma membrane of epidermal cells of onion Allium sp. and in chloroplasts of leaves of Nicotiana benthaminana. The coding region of IbGGPS is cloned into a binary vector under the control of 35S promoter and then transformed into Arabidopsis thaliana ecotype Columbia-0 to obtain transgenic plants. Transformations are made using Agrobacterium tumefaciens strain EHA105, semi- and quantitative real-time PCR expression analysis | Ipomoea batatas |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of the sweetpotato geranylgeranyl pyrophosphate synthase gene IbGGPS in Arabidopsis thaliana increases carotenoid content and enhances osmotic stress tolerance in the transgenic plants. Transgenic seedlings grow significantly longer roots compared to wild-type. Quantitative RT-PCR analysis shows altered expressions of several genes involved in the carotenoid biosynthesis in transgenic plants | Ipomoea batatas |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Ipomoea batatas | 9507 | - |
plasma membrane | - |
Ipomoea batatas | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ipomoea batatas | G8XQT1 | cv. Nongdafu 14, gene IbGGPS | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | analysis of enzyme expression tissue distribution by quantitative real-time PCR | Ipomoea batatas | - |
storage root | - |
Ipomoea batatas | - |
Synonyms | Comment | Organism |
---|---|---|
geranylgeranyl pyrophosphate synthase | - |
Ipomoea batatas |
GGPS | - |
Ipomoea batatas |
IbGGPS | - |
Ipomoea batatas |
General Information | Comment | Organism |
---|---|---|
physiological function | the formation of geranylgeranyl diphosphate is a key step in biosynthetic pathway of carotenoids and many other terpenes. This step is catalyzed by geranylgeranyl diphosphate synthase. Expression of enzyme-encoding gene IbGGPS is likely associated with carotenoid profiles in storage roots. Sweet potato geranylgeranyl pyrophosphate synthase gene IbGGPS increases carotenoid content and enhances osmotic stress tolerance in Arabidopsis thaliana | Ipomoea batatas |