Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-hydroxydecane-1,1-bisphosphonate | IC50: 720 nM | Homo sapiens | |
digeranyl bisphosphonate | IC50: 200 nM | Homo sapiens | |
additional information | potency and specificity of bisphosphonate enzyme inhibitors | Homo sapiens | |
zoledronate | weak inhibition, IC50: 0.1 mM, above | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Zn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans,trans-farnesyl diphosphate + isopentenyl diphosphate | Homo sapiens | the enzyme participates in the mevalonate pathway, overview, enzyme inhibition leads to enhanced depletion of intracellular geranylgeranyl diphosphate relative to the nitrogenous bisphosphonate, overview | diphosphate + geranylgeranyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
RPMI-8226 cell | myeloma cell line | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.133 | - |
purified recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans,trans-farnesyl diphosphate + isopentenyl diphosphate | - |
Homo sapiens | diphosphate + geranylgeranyl diphosphate | - |
? | |
trans,trans-farnesyl diphosphate + isopentenyl diphosphate | the enzyme participates in the mevalonate pathway, overview, enzyme inhibition leads to enhanced depletion of intracellular geranylgeranyl diphosphate relative to the nitrogenous bisphosphonate, overview | Homo sapiens | diphosphate + geranylgeranyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
geranylgeranyl pyrophosphate synthase | - |
Homo sapiens |
GGPP synthase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0002 | - |
IC50: 200 nM | Homo sapiens | digeranyl bisphosphonate | |
0.00072 | - |
IC50: 720 nM | Homo sapiens | 1-hydroxydecane-1,1-bisphosphonate | |
0.1 | - |
weak inhibition, IC50: 0.1 mM, above | Homo sapiens | zoledronate |