Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.29 extracted from

  • Wiemer, A.J.; Tong, H.; Swanson, K.M.; Hohl, R.J.
    Digeranyl bisphosphonate inhibits geranylgeranyl pyrophosphate synthase (2007), Biochem. Biophys. Res. Commun., 353, 921-925.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-hydroxydecane-1,1-bisphosphonate IC50: 720 nM Homo sapiens
digeranyl bisphosphonate IC50: 200 nM Homo sapiens
additional information potency and specificity of bisphosphonate enzyme inhibitors Homo sapiens
zoledronate weak inhibition, IC50: 0.1 mM, above Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens
Zn2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans,trans-farnesyl diphosphate + isopentenyl diphosphate Homo sapiens the enzyme participates in the mevalonate pathway, overview, enzyme inhibition leads to enhanced depletion of intracellular geranylgeranyl diphosphate relative to the nitrogenous bisphosphonate, overview diphosphate + geranylgeranyl diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
RPMI-8226 cell myeloma cell line Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.133
-
purified recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans,trans-farnesyl diphosphate + isopentenyl diphosphate
-
Homo sapiens diphosphate + geranylgeranyl diphosphate
-
?
trans,trans-farnesyl diphosphate + isopentenyl diphosphate the enzyme participates in the mevalonate pathway, overview, enzyme inhibition leads to enhanced depletion of intracellular geranylgeranyl diphosphate relative to the nitrogenous bisphosphonate, overview Homo sapiens diphosphate + geranylgeranyl diphosphate
-
?

Synonyms

Synonyms Comment Organism
geranylgeranyl pyrophosphate synthase
-
Homo sapiens
GGPP synthase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0002
-
IC50: 200 nM Homo sapiens digeranyl bisphosphonate
0.00072
-
IC50: 720 nM Homo sapiens 1-hydroxydecane-1,1-bisphosphonate
0.1
-
weak inhibition, IC50: 0.1 mM, above Homo sapiens zoledronate