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Literature summary for 2.5.1.29 extracted from

  • Laskaris, G.; van der Heijden, R.; Verpoorte, R.
    Purification and partial characterization of geranylgeranyl diphosphate synthase, from Taxus baccata cell cultures. An enzyme that regulates taxane biosynthesis (2000), Plant Sci., 153, 97-105.
    View publication on PubMed
Search for more literature for 2.5.1.29

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 farnesyl diphosphate
-
 Ricinus communis
0.0015
-
 isopentenyl diphosphate
-
 Taxus baccata
0.00246
-
 farnesyl diphosphate
-
 Taxus baccata
0.0035
-
 isopentenyl diphosphate
-
 Ricinus communis
0.0127
-
 dimethylallyl diphosphate
-
 Taxus baccata

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information enzyme seems to be membrane associated Taxus baccata
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Taxus baccata
Mg2+ maximal activity at 2 mM together with Mn2+ at 3 mM Taxus baccata
Mn2+ required for activity Taxus baccata
Mn2+ most effective divalent cation, maximal activity at 3 mM Taxus baccata
Zn2+ least effective divalent cation Taxus baccata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000, SDS-PAGE Taxus baccata
72000
-
 gel filtration Ricinus communis
76000
-
 gel filtration Taxus baccata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Ricinus communis no activity with dimethylallyl diphosphate geranylgeranyl diphosphate + diphosphate
-
 ?
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Taxus baccata enzyme regulates taxane biosynthesis geranylgeranyl diphosphate + diphosphate
-
 ?
dimethylallyl diphosphate + 3 isopentenyl diphosphate Taxus baccata low activity geranylgeranyl diphosphate + 3 diphosphate
-
 ?
geranyl diphosphate + 2 isopentenyl diphosphate Taxus baccata
-
 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
-
-
Taxus baccata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, phenyl Sepharose 6, Mono Q, Tosohaas-G3000, Mono P Taxus baccata

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Taxus baccata
-
seedling
-
 Ricinus communis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.8
-
-
 Taxus baccata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Taxus baccata geranylgeranyl diphosphate + diphosphate
-
 ?
(E,E)-farnesyl diphosphate + isopentenyl diphosphate no activity with dimethylallyl diphosphate Ricinus communis geranylgeranyl diphosphate + diphosphate
-
 ?
(E,E)-farnesyl diphosphate + isopentenyl diphosphate enzyme regulates taxane biosynthesis Taxus baccata geranylgeranyl diphosphate + diphosphate
-
 ?
dimethylallyl diphosphate + 3 isopentenyl diphosphate low activity Taxus baccata geranylgeranyl diphosphate + 3 diphosphate
-
 ?
geranyl diphosphate + 2 isopentenyl diphosphate
-
 Taxus baccata geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Taxus baccata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 7.2
-
 Taxus baccata