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Literature summary for 2.5.1.29 extracted from

  • Laferriere, A.; Beyer, P.
    Purification of geranylgeranyl diphosphate synthase from Sinapis alba etioplasts (1991), Biochim. Biophys. Acta, 1077, 167-172.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Aminophenylethyl diphosphate
-
Sinapis alba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
dimethylallyl diphosphate
-
Sinapis alba
0.033
-
isopentenyl diphosphate
-
Sinapis alba

Localization

Localization Comment Organism GeneOntology No. Textmining
etioplast
-
Sinapis alba 9513
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, SDS-PAGE Sinapis alba
74000
-
gel filtration Sinapis alba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Sinapis alba
-
geranylgeranyl diphosphate + diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate Sinapis alba
-
geranylgeranyl diphosphate + 2 diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Sinapis alba
-
mustard
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange chromatography, aminophenylethyl diphosphate affinity column Sinapis alba

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
4300000 cpm/mg Sinapis alba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
Sinapis alba geranylgeranyl diphosphate + diphosphate
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
Sinapis alba geranylgeranyl diphosphate + 3 diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate
-
Sinapis alba geranylgeranyl diphosphate + 2 diphosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Sinapis alba