Cloned (Comment) | Organism |
---|---|
cDNA encoding BoAIPT1, DNA and amino acid sequence determination and analysis, sequence comparison, functional expression of N-terminally His6-tagged BoAIPT1 in Escherichia coli strain BL21 (DE3), optimum protein expression is obtained by incubating the IPTG-induced culture at 18°C for 18 hr, transgenic expression in bamboo plants, real-time PCR expression analysis | Bambusa oldhamii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bambusa oldhamii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, recombinant His6-tagged BoAIPT1, SDS-PAGE | Bambusa oldhamii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + AMP | Bambusa oldhamii | - |
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bambusa oldhamii | D7R812 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged BoAIPT1 from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and ultrafiltration | Bambusa oldhamii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | - |
Bambusa oldhamii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + AMP | - |
Bambusa oldhamii | diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, recombinant His6-tagged BoAIPT1, SDS-PAGE | Bambusa oldhamii |
Synonyms | Comment | Organism |
---|---|---|
adenylate isopentenyltransferase | - |
Bambusa oldhamii |
AIPT1 | - |
Bambusa oldhamii |
isopentenyltransferase | - |
Bambusa oldhamii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bambusa oldhamii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bambusa oldhamii |
General Information | Comment | Organism |
---|---|---|
additional information | transgenic expression of BoAIPT1 in bamboo plants increases the green bamboo shoot growth, with the top green shoot section having the highest expression level | Bambusa oldhamii |