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Literature summary for 2.5.1.27 extracted from

  • Brugiere, N.; Humbert, S.; Rizzo, N.; Bohn, J.; Habben, J.E.
    A member of the maize isopentenyl transferase gene family, Zea mays isopentenyl transferase 2 (ZmIPT2), encodes a cytokinin biosynthetic enzyme expressed during kernel development. Cytokinin biosynthesis in maize (2008), Plant Mol. Biol., 67, 215-229.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
construct consisting of the 35S promoter fused to the first intron of the maize alcohol dehydrogenase gene, fused to the isoform IPT2 coding sequence and a terminator. Ectopic expression in Arabidopsis thaliana Zea mays

Protein Variants

Protein Variants Comment Organism
additional information ectopic expression of isoform IPT2 in calli and in planta of Arabidopsis thaliana creates phenotypes consistent with cytokinin overproduction Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays B2XWS4 isoform isopentenyl transferase 2, IPT2
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Source Tissue

Source Tissue Comment Organism Textmining
embryo at 8-10 days after pollination the endosperm and especially the basal transfer cell layer is a major site of IPT2 expression, this expression persists in the basal transfer cell layer and the developing embryo into later kernel development stages Zea mays
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kernel strong expression of isoform IPT2 in developing kernels. At 8-10 days after pollination the endosperm and especially the basal transfer cell layer is a major site of IPT2 expression, this expression persists in the basal transfer cell layer and the developing embryo into later kernel development stages Zea mays
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leaf very low level of isoform IPT2 protein Zea mays
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root very low level of isoform IPT2 protein Zea mays
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stem very low level of isoform IPT2 protein Zea mays
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + ADP ADP and ATP are preferred over AMP Zea mays diphosphate + N-(dimethylallyl)adenosine 5'-diphosphate
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?
dimethylallyl diphosphate + AMP ADP and ATP are preferred over AMP Zea mays diphosphate + N-(dimethylallyl)adenosine 5'-phosphate
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dimethylallyl diphosphate + ATP ADP and ATP are preferred over AMP Zea mays diphosphate + N-(dimethylallyl)adenosine 5'-triphosphate
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additional information no substrate: 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate Zea mays ?
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?