Any feedback?
Literature summary for 2.5.1.22 extracted from
- Polyamine synthesis in mammalian tissues. Isolation and characterization of spermine synthase from bovine brain (1979), Eur. J. Biochem., 101, 619-626.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-methylthioadenosine | strong | Bos taurus |  |
| additional information | no inhibition by erythro-9-(2-hydroxy-3-nonyl)adenine, methylglyoxal-bis-(guanylhydrazone), aminoguanidine, guanethidine, L-canaline, MgCl2, KCl, diamines, polyamines, polyamine derivatives | Bos taurus | |
| N-ethylmaleimide | in the absence of sulfhydryl compounds | Bos taurus | |
| PCMB | in the absence of sulfhydryl compounds | Bos taurus | |
| putrescine | - |
Bos taurus | |
| S-adenosyl-L-homocysteine | not | Bos taurus | |
| S-adenosyl-L-methionine | not | Bos taurus | |
| spermine | product inhibition, weak | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
S-adenosylmethioninamine | - |
Bos taurus | |
| 0.006 | - |
spermidine | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metal ion requirement | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000, SDS-PAGE | Bos taurus |
| 88000 | 90000 | brain, gel filtration, sedimentation equilibrium centrifugation | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosylmethioninamine + spermidine | Bos taurus | involved in polyamine biosynthetic pathway | 5'-methylthioadenosine + spermine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| spermine-Sepharose affinity chromatography | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.396 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | overview | Bos taurus | ? | - |
? | |
| S-adenosylmethioninamine + spermidine | strictly specific for spermidine | Bos taurus | 5'-methylthioadenosine + spermine | - |
? | |
| S-adenosylmethioninamine + spermidine | involved in polyamine biosynthetic pathway | Bos taurus | 5'-methylthioadenosine + spermine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 45000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.1, brain | Bos taurus |
| 7 | 8 | broad | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor requirement | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
5'-methylthioadenosine | - |
Bos taurus | |
| 0.0017 | - |
putrescine | - |
Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
