Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.21 extracted from

  • Filiz, E.; Ozyigit, I.; Vatansever, R.
    Comparative analyses of squalene synthase (SQS) proteins in poplar and pine by using bioinformatics tools (2016), Tree Genet. Genomes, 12, 32.
No PubMed abstract available
Search for more literature for 2.5.1.21

Cloned(Commentary)

Cloned (Comment) Organism
gene PmSQS, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis Pinus massoniana
gene PtSQS, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis Populus trichocarpa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Populus trichocarpa
Mg2+ required Pinus massoniana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46600
-
-
 Pinus massoniana
47300
-
-
 Populus trichocarpa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ Populus trichocarpa
-
 squalene + 2 diphosphate + NADP+
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ Pinus massoniana
-
 squalene + 2 diphosphate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Pinus massoniana W6D7A6
-
-
Populus trichocarpa B9HRH0 gene PtSQS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 (2E,6E)-farnesyl diphosphate first reaction Populus trichocarpa diphosphate + presqualene diphosphate
-
 ?
2 (2E,6E)-farnesyl diphosphate first reaction Pinus massoniana diphosphate + presqualene diphosphate
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+
-
 Populus trichocarpa squalene + 2 diphosphate + NADP+
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+
-
 Pinus massoniana squalene + 2 diphosphate + NADP+
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ farnesyl diphosphate forms hydrogen bonds with enzyme residues Arg49, Arg74, Ser48, and Val47 of conserved 77DTVED81 motif Pinus massoniana squalene + 2 diphosphate + NADP+
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ farnesyl diphosphate forms hydrogen bonds with enzyme residues Asp81, Asp217, Glu80, and Gln206 of conserved 77DTVED81 and 213DYLED217 motifs Populus trichocarpa squalene + 2 diphosphate + NADP+
-
 ?
presqualene diphosphate + NAD(P)H + H+ second reaction Populus trichocarpa squalene + diphosphate + NAD(P)+
-
 ?
presqualene diphosphate + NAD(P)H + H+ second reaction Pinus massoniana squalene + diphosphate + NAD(P)+
-
 ?

Subunits

Subunits Comment Organism
? x * 46600, about, sequence calculation Pinus massoniana
? x * 47300, about, sequence calculation Populus trichocarpa

Synonyms

Synonyms Comment Organism
SQS
-
 Populus trichocarpa
SQS
-
 Pinus massoniana

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Populus trichocarpa
NADPH
-
 Pinus massoniana

pI Value

Organism Comment pI Value Maximum pI Value
Populus trichocarpa sequence calculation
-
 6.86
Pinus massoniana sequence calculation
-
 7.92

General Information

General Information Comment Organism
evolution phylogenetic analysis of SQS enzymes in plants shows highly similar conserved pattern including 77DTVED81 and 213DYLED217 motifs, which are rich in aspartic acids involved in FPP binding Populus trichocarpa
evolution phylogenetic analysis of SQS enzymes in plants shows highly similar conserved pattern including 77DTVED81 and 213DYLED217 motifs, which are rich in aspartic acids involved in FPP binding Pinus massoniana
additional information molecular docking study, overview Populus trichocarpa
additional information molecular docking study, overview Pinus massoniana
physiological function the enzyme squalene synthase catalyzes the first committed step in sterol biosynthesis by condensing two molecules of farnesyl diphosphate into squalene in two reaction steps Populus trichocarpa
physiological function the enzyme squalene synthase catalyzes the first committed step in sterol biosynthesis by condensing two molecules of farnesyl diphosphate into squalene in two reaction steps Pinus massoniana