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Literature summary for 2.5.1.21 extracted from

  • Furubayashi, M.; Li, L.; Katabami, A.; Saito, K.; Umeno, D.
    Directed evolution of squalene synthase for dehydrosqualene biosynthesis (2014), FEBS Lett., 588, 3375-3381.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli codon-optimized gene tsqs, recombinant expression of wild-type and endgineered enzymes in Escherichia coli strain XL1-Blue, coexpression with gene crtN encoding DSQ desaturase from Staphylococcus aureus in Escherichia coli results in carotinoid production and accumulation of C30 carotenoid pigments, which does not happen with coexpression of gene crtI encoding Pantoea ananatis phytoene desaturase, carotenoid pigment analysis, overview Thermosynechococcus vestitus
gene hsqs, recombinant expression of a truncated Q33R/D34N/S38N mutant lacking 30 N-terminal residues and 47 C-terminal residues in Escherichia coli strain XL1-Blue, coexpression with gene crtN encoding DSQ desaturase from Staphylococcus aureus in Escherichia coli results in carotinoid production and accumulation of C30 carotenoid pigments, which does not happen with coexpression of gene crtI encoding Pantoea ananatis phytoene desaturase, carotenoid pigment analysis, overview Homo sapiens
gene ysqs, recombinant expression of wild-type and endgineered enzymes in Escherichia coli strain XL1-Blue, coexpression with gene crtN encoding DSQ desaturase from Staphylococcus aureus in Escherichia coli results in carotinoid production and accumulation of C30 carotenoid pigments, which does not happen with coexpression of gene crtI encoding Pantoea ananatis phytoene desaturase, carotenoid pigment analysis, overview Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information construction of a mutant containing three synonymous mutations G1125A, T1128C, T1176C and one nonsynonymous mutation G856A, which corresponds to Gly286Ser exchange, by gene replacement. Various mutations clustered around the residues that are important for NADPH binding effectively convert SQS into a dehydrosqualene synthase, overview Saccharomyces cerevisiae
additional information various mutations clustered around the residues that are important for NADPH binding effectively convert SQS into a dehydrosqualene synthase, overview Thermosynechococcus vestitus
additional information various mutations clustered around the residues that are important for NADPH binding effectively convert SQS into a dehydrosqualene synthase, overview Homo sapiens
Q33R/D34N/S38N the mutations do not affect the enzyme activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P37268 gene hsqs
-
Saccharomyces cerevisiae P53866 gene ysqs
-
Thermosynechococcus vestitus
-
gene tsqs
-

Synonyms

Synonyms Comment Organism
Erg9
-
Saccharomyces cerevisiae
SQS
-
Thermosynechococcus vestitus
SQS
-
Homo sapiens
SQS
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism squalene synthase catalyzes the first step of sterol/hopanoid biosynthesis in the organism Thermosynechococcus vestitus
metabolism squalene synthase catalyzes the first step of sterol/hopanoid biosynthesis in the organism Homo sapiens
metabolism squalene synthase catalyzes the first step of sterol/hopanoid biosynthesis in the organism Saccharomyces cerevisiae