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Literature summary for 2.5.1.21 extracted from

  • Grover, A.; Samuel, G.; Bisaria, V.S.; Sundar, D.
    Enhanced withanolide production by overexpression of squalene synthase in Withania somnifera (2013), J. Biosci. Bioeng., 115, 680-685.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning in Escherichia coli strain DH5alpha, overexpression of the endogenoous gene encoding squalene synthase in Withania somnifera via transfection with Agrobacterium tumefaciens strain GV3101 using binary vector pBI121 Withania somnifera

Organism

Organism UniProt Comment Textmining
Withania somnifera
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-
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Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
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Withania somnifera
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leaf
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Withania somnifera
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seedling
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Withania somnifera
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shoot
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Withania somnifera
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General Information

General Information Comment Organism
additional information enzyme overexpression leads a significant 4fold enhancement in squalene synthase activity and 2.5fold enhancement in withanolide A content, transformed cell suspension cultures also produce withaferin A, which is absent in the non-transformed cell cultures Withania somnifera