Cloned (Comment) | Organism |
---|---|
cloning in Escherichia coli strain DH5alpha, overexpression of the endogenoous gene encoding squalene synthase in Withania somnifera via transfection with Agrobacterium tumefaciens strain GV3101 using binary vector pBI121 | Withania somnifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Withania somnifera | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Withania somnifera | - |
leaf | - |
Withania somnifera | - |
seedling | - |
Withania somnifera | - |
shoot | - |
Withania somnifera | - |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme overexpression leads a significant 4fold enhancement in squalene synthase activity and 2.5fold enhancement in withanolide A content, transformed cell suspension cultures also produce withaferin A, which is absent in the non-transformed cell cultures | Withania somnifera |