Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10 mM, not as effective as dithiothreitol | Hevea brasiliensis | |
dithiothreitol | 10 mM, required for maximal stability | Hevea brasiliensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.1-1 mM, complete inhibition | Hevea brasiliensis | |
diphosphate | - |
Hevea brasiliensis | |
iodoacetamide | complete inhibition | Hevea brasiliensis | |
iodoacetic acid | - |
Hevea brasiliensis | |
Sodium diphosphate | - |
Hevea brasiliensis | |
Sodium phosphate | - |
Hevea brasiliensis | |
Thiol alkylating agents | - |
Hevea brasiliensis | |
Zn2+ | 0.1-1 mM, complete inhibition | Hevea brasiliensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | maximal stimulation at 5 mM | Hevea brasiliensis | |
Mg2+ | required for activity | Hevea brasiliensis | |
Mg2+ | maximal stimulation at 1 mM | Hevea brasiliensis | |
Mn2+ | stimulates, maximal activity at 1 mM | Hevea brasiliensis | |
Pb2+ | maximal stimulation at 5 mM | Hevea brasiliensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Hevea brasiliensis |
70000 | - |
gel filtration | Hevea brasiliensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hevea brasiliensis | - |
- |
- |
Oxidation Stability | Organism |
---|---|
thiols stabilize | Hevea brasiliensis |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DE52, Sephacryl S-200, hexyl-Sepharose, Mono Q | Hevea brasiliensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
latex | - |
Hevea brasiliensis | - |
leaf | - |
Hevea brasiliensis | - |
Storage Stability | Organism |
---|---|
-80°C, 25 mM Tris-HCl, pH 8, 10 mM dithiothreitol, 1 mM MgSO4, 30% glycerol, 0.1% sodium azide, at least 6 months, no loss of activity | Hevea brasiliensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + isopentenyl diphosphate | - |
Hevea brasiliensis | (E,E)-farnesyl diphosphate + (E)-geranyl diphosphate | in the absence of rubber | ? | |
isopentenyl diphosphate + cis-1,4-polyisoprene | of rubber particles | Hevea brasiliensis | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Hevea brasiliensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Hevea brasiliensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
Sodium diphosphate | - |
Hevea brasiliensis | |
200 | - |
diphosphate | - |
Hevea brasiliensis |