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Literature summary for 2.5.1.20 extracted from

  • Light, D.R.; Dennis, M.S.
    Purification of a prenyltransferase that elongates cis-polyisoprene rubber from the latex of Hevea brasiliensis (1989), J. Biol. Chem., 264, 18589-18597.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 10 mM, not as effective as dithiothreitol Hevea brasiliensis
dithiothreitol 10 mM, required for maximal stability Hevea brasiliensis

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.1-1 mM, complete inhibition Hevea brasiliensis
diphosphate
-
Hevea brasiliensis
iodoacetamide complete inhibition Hevea brasiliensis
iodoacetic acid
-
Hevea brasiliensis
Sodium diphosphate
-
Hevea brasiliensis
Sodium phosphate
-
Hevea brasiliensis
Thiol alkylating agents
-
Hevea brasiliensis
Zn2+ 0.1-1 mM, complete inhibition Hevea brasiliensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ maximal stimulation at 5 mM Hevea brasiliensis
Mg2+ required for activity Hevea brasiliensis
Mg2+ maximal stimulation at 1 mM Hevea brasiliensis
Mn2+ stimulates, maximal activity at 1 mM Hevea brasiliensis
Pb2+ maximal stimulation at 5 mM Hevea brasiliensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, SDS-PAGE Hevea brasiliensis
70000
-
gel filtration Hevea brasiliensis

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis
-
-
-

Oxidation Stability

Oxidation Stability Organism
thiols stabilize Hevea brasiliensis

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DE52, Sephacryl S-200, hexyl-Sepharose, Mono Q Hevea brasiliensis

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
Hevea brasiliensis
-
leaf
-
Hevea brasiliensis
-

Storage Stability

Storage Stability Organism
-80°C, 25 mM Tris-HCl, pH 8, 10 mM dithiothreitol, 1 mM MgSO4, 30% glycerol, 0.1% sodium azide, at least 6 months, no loss of activity Hevea brasiliensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + isopentenyl diphosphate
-
Hevea brasiliensis (E,E)-farnesyl diphosphate + (E)-geranyl diphosphate in the absence of rubber ?
isopentenyl diphosphate + cis-1,4-polyisoprene of rubber particles Hevea brasiliensis diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
?

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Hevea brasiliensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Hevea brasiliensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5
-
Sodium diphosphate
-
Hevea brasiliensis
200
-
diphosphate
-
Hevea brasiliensis