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Literature summary for 2.5.1.19 extracted from
- Complementary screening, identification and application of a novel class II 5-enopyruvylshikimate-3-phosphate synthase from Bacillus cereus (2012), World J. Microbiol. Biotechnol., 29, 549-557.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | transgenic Arabidopsis thaliana plants expressing the enzyme from Bacillus cereus germinate well in presence of 1 mM glyphosate, while control plants do not germinate at 0.2 mM | Bacillus cereus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus cereus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glyphosate | - |
Bacillus cereus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.195 | - |
phosphoenolpyruvate | pH 7.0, 28°C | Bacillus cereus | |
| 0.304 | - |
3-phosphoshikimate | pH 7.0, 28°C | Bacillus cereus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43350 | - |
x * 43350, calculated | Bacillus cereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | F5A7Q0 | class II enzyme | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + 3-phosphoshikimate | - |
Bacillus cereus | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43350, calculated | Bacillus cereus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 31.8 | - |
phosphoenolpyruvate | pH 7.0, 28°C | Bacillus cereus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.052 | - |
glyphosate | pH 7.0, 28°C | Bacillus cereus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.458 | - |
pH 7.0, 28°C | Bacillus cereus | glyphosate | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 163.1 | - |
phosphoenolpyruvate | pH 7.0, 28°C | Bacillus cereus | |
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Release 2023.1 (January 2023)
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