Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.19 extracted from

  • Eschenburg, S.; Kabsch, W.; Healy, M.L.; Schonbrunn, E.
    A new view of the mechanisms of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) and 5-enolpyruvylshikimate-3-phosphate synthase (AroA) derived from X-ray structures of their tetrahedral reaction intermediate states (2003), J. Biol. Chem., 278, 49215-49222.
    View publication on PubMed

Application

Application Comment Organism
synthesis enzyme is a target for development and synthesis of antimicrobial drugs Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type and mutant enzymes in strain BL21(DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant wild-type and mutant enzymes in complex with the substrates, at 19°C, from 4 M sodium formate in presence of 5 mM 3-phosphoshikimate and 5 mM phosphoenolpyruvate, X-ray diffraction structure determination and analysis at 1.6 A resolution Escherichia coli

Protein Variants

Protein Variants Comment Organism
D313A site-directed mutagenesis, comparison of the mutant active site and substrate binding structures to those of the wild-type enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate Escherichia coli 6th enzyme in the shikimate pathway phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A6D3 gene aroA
-

Reaction

Reaction Comment Organism Reaction ID
phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate catalytic mechanism, tetrahedral reaction intermediate, active site structure, wild-type and mutant D313A Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate 6th enzyme in the shikimate pathway Escherichia coli phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
?
phosphoenolpyruvate + 3-phosphoshikimate substrate binding of wild-type and mutant D313A, overview Escherichia coli phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
?

Synonyms

Synonyms Comment Organism
5-enolpyruvylshikimate-3-phosphate synthase
-
Escherichia coli
AroA
-
Escherichia coli
More enzyme belongs to the family of enolpyruvyl transferases Escherichia coli