Application | Comment | Organism |
---|---|---|
synthesis | enzyme is a target for development and synthesis of antimicrobial drugs | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes in strain BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and mutant enzymes in complex with the substrates, at 19°C, from 4 M sodium formate in presence of 5 mM 3-phosphoshikimate and 5 mM phosphoenolpyruvate, X-ray diffraction structure determination and analysis at 1.6 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D313A | site-directed mutagenesis, comparison of the mutant active site and substrate binding structures to those of the wild-type enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | Escherichia coli | 6th enzyme in the shikimate pathway | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6D3 | gene aroA | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | catalytic mechanism, tetrahedral reaction intermediate, active site structure, wild-type and mutant D313A | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | 6th enzyme in the shikimate pathway | Escherichia coli | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | substrate binding of wild-type and mutant D313A, overview | Escherichia coli | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-enolpyruvylshikimate-3-phosphate synthase | - |
Escherichia coli |
AroA | - |
Escherichia coli |
More | enzyme belongs to the family of enolpyruvyl transferases | Escherichia coli |