Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4Cl | strong activation of forward reaction, effectiveness of activation in descending order: NH4Cl, Rb+, K+ | Streptococcus pneumoniae |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-phosphonomethylglycine | - |
Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00195 | - |
5-enolpyruvylshikimate 3-phosphate | reverse reaction, in the presence of 50 mM KCl | Streptococcus pneumoniae | |
0.022 | - |
phosphoenolpyruvate | in the presence of 100 mM NH4Cl | Streptococcus pneumoniae | |
0.023 | - |
phosphoenolpyruvate | in the presence of 1 mM NH4Cl and 100 mM KCl | Streptococcus pneumoniae | |
0.031 | - |
shikimate 3-phosphate | in the presence of 100 mM NH4Cl | Streptococcus pneumoniae | |
0.049 | - |
shikimate 3-phosphate | in the presence of 1 mM NH4Cl and 10 mM KCl | Streptococcus pneumoniae | |
0.085 | - |
phosphoenolpyruvate | in the presence of 1 mM NH4Cl and 10 mM KCl | Streptococcus pneumoniae | |
0.091 | - |
phosphoenolpyruvate | in the presence of 10 mM NH4Cl | Streptococcus pneumoniae | |
0.1 | - |
phosphoenolpyruvate | in the presence of 1 mM NH4Cl | Streptococcus pneumoniae | |
0.109 | - |
shikimate 3-phosphate | in the presence of 1 mM NH4Cl and 100 mM KCl | Streptococcus pneumoniae | |
0.118 | - |
shikimate 3-phosphate | in the presence of 10 mM NH4Cl | Streptococcus pneumoniae | |
0.145 | - |
shikimate 3-phosphate | in the presence of 1 mM NH4Cl | Streptococcus pneumoniae | |
0.343 | - |
phosphate | reverse reaction, in the presence of 50 mM KCl | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | strong activation of forward reaction, effectiveness of activation in descending order: NH4Cl, Rb+, K+ | Streptococcus pneumoniae | |
Rb+ | strong activation of forward reaction, effectiveness of activation in descending order: NH4Cl, Rb+, K+ | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | Q9S400 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, ammonium sulfate, hydrophobic interaction chromatography, anion-exchange chromatography | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Streptococcus pneumoniae | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
phosphate | reverse reaction, in the presence of 50 mM KCl | Streptococcus pneumoniae | |
0.23 | - |
5-enolpyruvylshikimate 3-phosphate | reverse reaction, in the presence of 50 mM KCl | Streptococcus pneumoniae | |
2 | - |
phosphoenolpyruvate | in the presence of 1 mM NH4Cl and 10 mM KCl | Streptococcus pneumoniae | |
2.1 | - |
phosphoenolpyruvate | in the presence of 1 mM NH4Cl | Streptococcus pneumoniae | |
2.8 | - |
phosphoenolpyruvate | in the presence of 10 mM NH4Cl | Streptococcus pneumoniae | |
3 | - |
phosphoenolpyruvate | in the presence of 100 mM NH4Cl | Streptococcus pneumoniae | |
12.8 | - |
phosphoenolpyruvate | in the presence of 1 mM NH4Cl and 100 mM KCl | Streptococcus pneumoniae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
N-phosphonomethylglycine | in the presence of 100 mM NH4Cl | Streptococcus pneumoniae | |
0.005 | - |
N-phosphonomethylglycine | in the presence of 1 mM NH4Cl and 100 mM K+ | Streptococcus pneumoniae | |
0.098 | - |
N-phosphonomethylglycine | in the presence of 1 mM NH4Cl and 10 mM K+ | Streptococcus pneumoniae | |
0.185 | - |
N-phosphonomethylglycine | in the presence of 1 mM NH4Cl | Streptococcus pneumoniae |