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Literature summary for 2.5.1.19 extracted from

  • Du, W.; Wallis, N.G.; Mazzulla, M.J.; Chalker, A.F.; Zhang, L.; Liu, W.S.; Kallender, H.; Payne, D.J.
    Characterization of Streptococcus pneumoniae 5-enolpyruvylshikimate 3-phosphate synthase and its activation by univalent cations (2000), Eur. J. Biochem., 267, 222-227.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NH4Cl strong activation of forward reaction, effectiveness of activation in descending order: NH4Cl, Rb+, K+ Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
N-phosphonomethylglycine
-
Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00195
-
5-enolpyruvylshikimate 3-phosphate reverse reaction, in the presence of 50 mM KCl Streptococcus pneumoniae
0.022
-
phosphoenolpyruvate in the presence of 100 mM NH4Cl Streptococcus pneumoniae
0.023
-
phosphoenolpyruvate in the presence of 1 mM NH4Cl and 100 mM KCl Streptococcus pneumoniae
0.031
-
shikimate 3-phosphate in the presence of 100 mM NH4Cl Streptococcus pneumoniae
0.049
-
shikimate 3-phosphate in the presence of 1 mM NH4Cl and 10 mM KCl Streptococcus pneumoniae
0.085
-
phosphoenolpyruvate in the presence of 1 mM NH4Cl and 10 mM KCl Streptococcus pneumoniae
0.091
-
phosphoenolpyruvate in the presence of 10 mM NH4Cl Streptococcus pneumoniae
0.1
-
phosphoenolpyruvate in the presence of 1 mM NH4Cl Streptococcus pneumoniae
0.109
-
shikimate 3-phosphate in the presence of 1 mM NH4Cl and 100 mM KCl Streptococcus pneumoniae
0.118
-
shikimate 3-phosphate in the presence of 10 mM NH4Cl Streptococcus pneumoniae
0.145
-
shikimate 3-phosphate in the presence of 1 mM NH4Cl Streptococcus pneumoniae
0.343
-
phosphate reverse reaction, in the presence of 50 mM KCl Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
K+ strong activation of forward reaction, effectiveness of activation in descending order: NH4Cl, Rb+, K+ Streptococcus pneumoniae
Rb+ strong activation of forward reaction, effectiveness of activation in descending order: NH4Cl, Rb+, K+ Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q9S400
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, ammonium sulfate, hydrophobic interaction chromatography, anion-exchange chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Streptococcus pneumoniae phosphate + 5-enolpyruvylshikimate 3-phosphate
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
phosphate reverse reaction, in the presence of 50 mM KCl Streptococcus pneumoniae
0.23
-
5-enolpyruvylshikimate 3-phosphate reverse reaction, in the presence of 50 mM KCl Streptococcus pneumoniae
2
-
phosphoenolpyruvate in the presence of 1 mM NH4Cl and 10 mM KCl Streptococcus pneumoniae
2.1
-
phosphoenolpyruvate in the presence of 1 mM NH4Cl Streptococcus pneumoniae
2.8
-
phosphoenolpyruvate in the presence of 10 mM NH4Cl Streptococcus pneumoniae
3
-
phosphoenolpyruvate in the presence of 100 mM NH4Cl Streptococcus pneumoniae
12.8
-
phosphoenolpyruvate in the presence of 1 mM NH4Cl and 100 mM KCl Streptococcus pneumoniae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0003
-
N-phosphonomethylglycine in the presence of 100 mM NH4Cl Streptococcus pneumoniae
0.005
-
N-phosphonomethylglycine in the presence of 1 mM NH4Cl and 100 mM K+ Streptococcus pneumoniae
0.098
-
N-phosphonomethylglycine in the presence of 1 mM NH4Cl and 10 mM K+ Streptococcus pneumoniae
0.185
-
N-phosphonomethylglycine in the presence of 1 mM NH4Cl Streptococcus pneumoniae