Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Cl- | at about 100 mM | Sorghum bicolor | |
F- | stimulation | Sorghum bicolor | |
NO3- | stimulation at low concentrations, inhibition above 15 mM | Sorghum bicolor | |
SO42- | slight stimulation, inhibition above 50 mM | Sorghum bicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium heptamolybdate | 1.1 mM, 50% inhibition, no inhibition below 0.1 mM | Sorghum bicolor | |
CaCl2 | above 100 mM | Sorghum bicolor | |
Cu2+ | 5 mM, complete inhibition | Sorghum bicolor | |
Fe2+ | 5 mM, 42% inhibition | Sorghum bicolor | |
Fe3+ | 5 mM, 68% inhibition | Sorghum bicolor | |
MgCl2 | above 100 mM | Sorghum bicolor | |
Mn2+ | 5 mM, 42% inhibition | Sorghum bicolor | |
N-phosphonomethylglycine | - |
Sorghum bicolor | |
Pb2+ | 5 mM, 97% inhibition | Sorghum bicolor | |
SO42- | inhibition above 50 mM, slight stimulation below | Sorghum bicolor | |
Zn2+ | 5 mM, complete inhibition | Sorghum bicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
3-phosphoshikimate | - |
Sorghum bicolor | |
0.008 | - |
phosphoenolpyruvate | - |
Sorghum bicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | stimulation at 20 mM, inhibition above 100 mM | Sorghum bicolor | |
MgCl2 | stimulation at 20 mM, inhibition above 100 mM | Sorghum bicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
gel filtration | Sorghum bicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, hydroxyapatite, phenylaggarose, Sephacryl S-200, isozymes I, II and III | Sorghum bicolor |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.1 | - |
- |
Sorghum bicolor |
Storage Stability | Organism |
---|---|
-20°C, 40% v/v glycerol, at least 1 year, no loss of activity | Sorghum bicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Sorghum bicolor | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50600-51900, 3 isozymes, SDS-PAGE | Sorghum bicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sorghum bicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
Tris-HCl buffer preferred | Sorghum bicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | approx. half-maximal activity at pH 6.5 and 8.5 | Sorghum bicolor |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00016 | - |
N-phosphonomethylglycine | vs. phosphoenolpyruvate | Sorghum bicolor |