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Literature summary for 2.5.1.18 extracted from
- Molecular cloning and biochemical characterization of a Tau class glutathione S-transferase from Pinus brutia Ten (2020), Trees, 34, 835-843 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pinus brutia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
Ethacrynic acid | at pH 6.5 and 25°C | Pinus brutia |  |
| 3.66 | - |
1-chloro-2,4-dinitrobenzene | at pH 6.5 and 25°C | Pinus brutia | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pinus brutia | D3YLT8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Pinus brutia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Pinus brutia | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
| glutathione + ethacrynic acid | - |
Pinus brutia | ? | - |
? | |
| additional information | no activity towards 1,2-dichloro-4-nitrobenzene | Pinus brutia | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27370, His6-tagged recombinant enzyme, calculated from amino acid sequence | Pinus brutia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GST Tau | - |
Pinus brutia |
| tau class glutathione S-transferase | - |
Pinus brutia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Pinus brutia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 55 | the enzyme retains 91% and 75% of its initial activity after 15 min incubation at 35°C and 45°C, respectively. The enzyme activity decreases to 12% of its maximum at 55°C | Pinus brutia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
with 1-chloro-2,4-dinitrobenzene as substrate | Pinus brutia |
| 8 | - |
with ethacrynic acid as substrate | Pinus brutia |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0018 | - |
1-chloro-2,4-dinitrobenzene | at pH 6.5 and 25°C | Pinus brutia | |
| 0.0225 | - |
Ethacrynic acid | at pH 6.5 and 25°C | Pinus brutia | |
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Release 2023.1 (January 2023)
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