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Literature summary for 2.5.1.18 extracted from

  • Kalita, J.; Shukla, R.; Shukla, H.; Gadhave, K.; Giri, R.; Tripathi, T.
    Comprehensive analysis of the catalytic and structural properties of a mu-class glutathione S-transferase from Fasciola gigantica (2017), Sci. Rep., 7, 17547 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Fasciola gigantica

Inhibitors

Inhibitors Comment Organism Structure
Cibacron blue
-
 Fasciola gigantica
additional information not inhibited by bromosulfophthalein Fasciola gigantica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00958
-
 trans-2-nonenal at pH 7.5 and 30°C Fasciola gigantica
0.01424
-
 trans-4-phenyl-3-butene-2-one at pH 7.5 and 30°C Fasciola gigantica
0.04835
-
 hexa-2,4-dienal at pH 7.5 and 30°C Fasciola gigantica
0.04835
-
 4-nitroquinoline-1-oxide at pH 7.5 and 30°C Fasciola gigantica
0.2313
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Fasciola gigantica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Fasciola gigantica

Organism

Organism UniProt Comment Textmining
Fasciola gigantica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GST affinity column chromatography Fasciola gigantica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.04
-
 with hexa-2,4-dienal as substrate, at pH 7.5 and 30°C Fasciola gigantica
4.54
-
 with trans-4-phenyl-3-butene-2-one as substrate, at pH 7.5 and 30°C Fasciola gigantica
5.1
-
 with trans-2-nonenal as substrate, at pH 7.5 and 30°C Fasciola gigantica
13.14
-
 with 4-nitroquinoline-1-oxide as substrate, at pH 7.5 and 30°C Fasciola gigantica
68.54
-
 with 1-chloro-2,4-dinitrobenzene as substrate, at pH 7.5 and 30°C Fasciola gigantica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Fasciola gigantica chloride + 2,4-dinitrophenyl-glutathione
-
 ?
glutathione + 4-nitroquinoline-1-oxide
-
 Fasciola gigantica ?
-
 ?
glutathione + hexa-2,4-dienal
-
 Fasciola gigantica ?
-
 ?
glutathione + trans-2-nonenal
-
 Fasciola gigantica ?
-
 ?
glutathione + trans-4-phenyl-3-butene-2-one
-
 Fasciola gigantica ?
-
 ?
additional information no activity against 1,2-dichloro-4-nitrobenzene and ethacrynic acid Fasciola gigantica ?
-
-

Subunits

Subunits Comment Organism
homodimer 2 * 25000, SDS-PAGE Fasciola gigantica

Synonyms

Synonyms Comment Organism
GST1
-
 Fasciola gigantica
mu-class glutathione S-transferase
-
 Fasciola gigantica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Fasciola gigantica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 the enzyme activity is reduced to approximately 50% at 20°C, to 25% at 60°C and 20% at 70°C Fasciola gigantica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.47
-
 hexa-2,4-dienal at pH 7.5 and 30°C Fasciola gigantica
3.9
-
 4-nitroquinoline-1-oxide at pH 7.5 and 30°C Fasciola gigantica
4.38
-
 trans-2-nonenal at pH 7.5 and 30°C Fasciola gigantica
11.28
-
 trans-4-phenyl-3-butene-2-one at pH 7.5 and 30°C Fasciola gigantica
58.77
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Fasciola gigantica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Fasciola gigantica

pH Range

pH Minimum pH Maximum Comment Organism
6 9 the enzyme is highly active between pH 6.0 and 9.0. At pH values below 6.0 and above 9.0, the activity decreases substantially Fasciola gigantica

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00135
-
 at pH 7.5 and 25°C Fasciola gigantica Cibacron blue

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
39.7
-
 hexa-2,4-dienal at pH 7.5 and 30°C Fasciola gigantica
233
-
 trans-4-phenyl-3-butene-2-one at pH 7.5 and 30°C Fasciola gigantica
254
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Fasciola gigantica
274
-
 4-nitroquinoline-1-oxide at pH 7.5 and 30°C Fasciola gigantica
457
-
 trans-2-nonenal at pH 7.5 and 30°C Fasciola gigantica