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Literature summary for 2.5.1.18 extracted from
- Cloning, characterization and expression analysis of glutathione S-transferase from the antarctic yeast Rhodotorula mucilaginosa AN5 (2020), Protein Expr. Purif., 167, 105518 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Rhodotorula mucilaginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
glutathione | at pH 7.0 and 37°C | Rhodotorula mucilaginosa |  |
| 0.4 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.0 and 37°C | Rhodotorula mucilaginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is almost unaffected by Hg2+ and Mn2+ | Rhodotorula mucilaginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula mucilaginosa | - |
- |
- |
| Rhodotorula mucilaginosa AN5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Rhodotorula mucilaginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
crude extract, at pH 7.0 and 25°C | Rhodotorula mucilaginosa |
| 60.2 | - |
after 5.73fold purification, at pH 7.0 and 25°C | Rhodotorula mucilaginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Rhodotorula mucilaginosa | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Rhodotorula mucilaginosa AN5 | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30400, calculated from amino acid sequence | Rhodotorula mucilaginosa |
| ? | x * 35100, recombinant enzyme, SDS-PAGE | Rhodotorula mucilaginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Rhodotorula mucilaginosa |
| GST | - |
Rhodotorula mucilaginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Rhodotorula mucilaginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | the enzyme is most stable at 25°C and 35°C for 45 min. Complete enzyme inactivation is observed after incubation at 55°C for 1 h | Rhodotorula mucilaginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rhodotorula mucilaginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | the enzyme is highly active at pH 6.0-8.0 | Rhodotorula mucilaginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | the enzyme shows maximal stability at pH 3.0, while its activity is significantly reduced after 45 min at pH 9.0 | Rhodotorula mucilaginosa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rhodotorula mucilaginosa | calculated from amino acid sequence | - |
5.4 |
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