Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodotorula mucilaginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
glutathione | at pH 7.0 and 37°C | Rhodotorula mucilaginosa | |
0.4 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.0 and 37°C | Rhodotorula mucilaginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is almost unaffected by Hg2+ and Mn2+ | Rhodotorula mucilaginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula mucilaginosa | - |
- |
- |
Rhodotorula mucilaginosa AN5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Rhodotorula mucilaginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.5 | - |
crude extract, at pH 7.0 and 25°C | Rhodotorula mucilaginosa |
60.2 | - |
after 5.73fold purification, at pH 7.0 and 25°C | Rhodotorula mucilaginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Rhodotorula mucilaginosa | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Rhodotorula mucilaginosa AN5 | chloride + 2,4-dinitrophenyl-glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30400, calculated from amino acid sequence | Rhodotorula mucilaginosa |
? | x * 35100, recombinant enzyme, SDS-PAGE | Rhodotorula mucilaginosa |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Rhodotorula mucilaginosa |
GST | - |
Rhodotorula mucilaginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Rhodotorula mucilaginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | the enzyme is most stable at 25°C and 35°C for 45 min. Complete enzyme inactivation is observed after incubation at 55°C for 1 h | Rhodotorula mucilaginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodotorula mucilaginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the enzyme is highly active at pH 6.0-8.0 | Rhodotorula mucilaginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | the enzyme shows maximal stability at pH 3.0, while its activity is significantly reduced after 45 min at pH 9.0 | Rhodotorula mucilaginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodotorula mucilaginosa | calculated from amino acid sequence | - |
5.4 |