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Literature summary for 2.5.1.18 extracted from

  • Hamed, R.; Maharem, T.; Guneidy, R.; Emam, M.; Abdel Karim, G.
    Purification of fat body glutathione S-transferase from the desert locust Schistocerca gregaria investigation of flavonoid inhibitory effects on enzyme activity (2019), Physiol. Entomol., 44, 187-199 .
No PubMed abstract available
Search for more literature for 2.5.1.18

Inhibitors

Inhibitors Comment Organism Structure
bromosulfophthalein
-
 Schistocerca gregaria
Cibacron blue most potent inhibitor Schistocerca gregaria
delphinidin chloride strong inhibition Schistocerca gregaria
Ethacrynic acid
-
 Schistocerca gregaria
additional information not inhibited by 18alpha-glycyrrhetinic acid and cyanidin Schistocerca gregaria
quercetin strong inhibition Schistocerca gregaria
rutin
-
 Schistocerca gregaria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 1-chloro-2,4-dinitrobenzene at pH 8.0 and 30°C Schistocerca gregaria
0.354
-
 glutathione at pH 8.0 and 30°C Schistocerca gregaria

Organism

Organism UniProt Comment Textmining
Schistocerca gregaria
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and GSH-Sepharose column chromatography Schistocerca gregaria

Source Tissue

Source Tissue Comment Organism Textmining
fat body highest enzyme activity in fat body Schistocerca gregaria
-
foregut
-
 Schistocerca gregaria
-
hindgut
-
 Schistocerca gregaria
-
midgut
-
 Schistocerca gregaria
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.66
-
 crude extract, at pH 8.0 and 30°C Schistocerca gregaria
42.8
-
 after 16.1fold purification, at pH 8.0 and 30°C Schistocerca gregaria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane
-
 Schistocerca gregaria ?
-
 ?
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Schistocerca gregaria chloride + 2,4-dinitrophenyl-glutathione
-
 ?
glutathione + cumene hydroperoxide 114% activity compared to 1-chloro-2,4-dinitrobenzene Schistocerca gregaria ?
-
 ?
additional information the enzyme has no detectable activity toward 1,2-dichloro-4-nitrobenzene, ethacrynic acid, bromosulfophthalein, 1,2-epoxy-3-(4-nitrophenoxy) propane, 4-nitrophenethyl bromide and styrene oxide Schistocerca gregaria ?
-
-

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Schistocerca gregaria
? x * 23600, calculated from amino acid sequence Schistocerca gregaria

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
 Schistocerca gregaria
GST
-
 Schistocerca gregaria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 optimum at pH 8.0 followed by an approximately 70% decrease in enzymatic activity in alkaline pH values Schistocerca gregaria

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000189
-
 at pH 8.0 and 30°C Schistocerca gregaria Cibacron blue
0.00223
-
 at pH 8.0 and 30°C Schistocerca gregaria quercetin
0.00388
-
 at pH 8.0 and 30°C Schistocerca gregaria delphinidin chloride
0.0103
-
 at pH 8.0 and 30°C Schistocerca gregaria rutin
0.363
-
 at pH 8.0 and 30°C Schistocerca gregaria bromosulfophthalein
3.63
-
 at pH 8.0 and 30°C Schistocerca gregaria Ethacrynic acid