Cloned (Comment) | Organism |
---|---|
expressed as His6-tagged protein in Escherichia coli BL21(DE3) cells | Novosphingobium aromaticivorans |
Protein Variants | Comment | Organism |
---|---|---|
N14A | the mutant of subunit A has a kcat value about 20fold lower and a Km value about 12.5fold higher than that of the wild type enzyme | Novosphingobium aromaticivorans |
S14A | the mutant of subunit B has the same kcat and Km values as the wild type enzyme | Novosphingobium aromaticivorans |
S14A/S15A | the double mutant has a kcat value similar to that of the wild type enzyme and a Km value that is 5-6fold higher than that of the wild type enzyme | Novosphingobium aromaticivorans |
S15A | the mutant of subunit A has a kcat value similar to that of the wild type enzyme and a Km value that is 5-6fold higher than that of the wild type enzyme | Novosphingobium aromaticivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | wild type enzyme, at pH 8.0 and 30°C | Novosphingobium aromaticivorans | |
0.022 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | mutant enzyme S14A, at pH 8.0 and 30°C | Novosphingobium aromaticivorans | |
0.1 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | mutant enzyme S15A, at pH 8.0 and 30°C | Novosphingobium aromaticivorans | |
0.12 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | mutant enzyme S14A/S15A, at pH 8.0 and 30°C | Novosphingobium aromaticivorans | |
0.25 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | mutant enzyme N14A, at pH 8.0 and 30°C | Novosphingobium aromaticivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
gel filtration | Novosphingobium aromaticivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Novosphingobium aromaticivorans | Q2G4B4 and Q2G4B5 | subunits BaeA and BaeB | - |
Novosphingobium aromaticivorans DSM 12444 | Q2G4B4 and Q2G4B5 | subunits BaeA and BaeB | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Novosphingobium aromaticivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | the enzyme stereospecifically cleaves the beta(R)-aryl ether bond of the di-aromatic compound beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | Novosphingobium aromaticivorans | beta-(S)-hydroxypropiovanillonyl-glutathione + guaiacol | - |
? | |
glutathione + beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | the enzyme stereospecifically cleaves the beta(R)-aryl ether bond of the di-aromatic compound beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | Novosphingobium aromaticivorans DSM 12444 | beta-(S)-hydroxypropiovanillonyl-glutathione + guaiacol | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 29400 + 1 * 27000, SDS-PAGE | Novosphingobium aromaticivorans |
Synonyms | Comment | Organism |
---|---|---|
BaeAB | - |
Novosphingobium aromaticivorans |
beta-etherase | - |
Novosphingobium aromaticivorans |
glutathione S-transferase | - |
Novosphingobium aromaticivorans |
GST | - |
Novosphingobium aromaticivorans |
Saro_2873/Saro_2872 | - |
Novosphingobium aromaticivorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | mutant enzyme N14A, at pH 8.0 and 30°C | Novosphingobium aromaticivorans | |
2.4 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | mutant enzyme S15A, at pH 8.0 and 30°C | Novosphingobium aromaticivorans | |
2.8 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | mutant enzyme S14A, at pH 8.0 and 30°C | Novosphingobium aromaticivorans | |
2.8 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | mutant enzyme S14A/S15A, at pH 8.0 and 30°C | Novosphingobium aromaticivorans | |
2.9 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | wild type enzyme, at pH 8.0 and 30°C | Novosphingobium aromaticivorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | mutant enzyme N14A, at pH 8.0 and 30°C | Novosphingobium aromaticivorans | |
23 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | mutant enzyme S14A/S15A, at pH 8.0 and 30°C | Novosphingobium aromaticivorans | |
23 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | mutant enzyme S15A, at pH 8.0 and 30°C | Novosphingobium aromaticivorans | |
130 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | mutant enzyme S14A, at pH 8.0 and 30°C | Novosphingobium aromaticivorans | |
150 | - |
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone | wild type enzyme, at pH 8.0 and 30°C | Novosphingobium aromaticivorans |