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Literature summary for 2.5.1.18 extracted from

  • Kalita, J.; Shukla, H.; Tripathi, T.
    Engineering glutathione S-transferase with a point mutation at conserved F136 residue increases the xenobiotic-metabolizing activity (2020), Int. J. Biol. Macromol., 163, 1117-1126 .
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Fasciola gigantica

Protein Variants

Protein Variants Comment Organism
F136A the mutation drastically increases the protein activity to more than 1.5fold. Both binding affinity and catalytic efficiency of the mutant enzyme to the substrates 1-chloro-2,4-dinitrobenzene as well as glutathione are increased compared to the wild type enzyme Fasciola gigantica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.305
-
 1-chloro-2,4-dinitrobenzene mutant enzyme F136A, at pH 7.5, temperature not specified in the publication Fasciola gigantica
0.402
-
 1-chloro-2,4-dinitrobenzene wild type enzyme, at pH 7.5, temperature not specified in the publication Fasciola gigantica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Fasciola gigantica

Organism

Organism UniProt Comment Textmining
Fasciola gigantica Q9XYL9
-
-

Purification (Commentary)

Purification (Comment) Organism
GSH-agarose bead chromatography and Superdex 200 gel filtration Fasciola gigantica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
105.45
-
 wild type enzyme, at pH 7.5, temperature not specified in the publication Fasciola gigantica
120.3
-
 mutant enzyme F136A, at pH 7.5, temperature not specified in the publication Fasciola gigantica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Fasciola gigantica chloride + S-(2,4-dinitrophenyl)-glutathione
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 25000, SDS-PAGE Fasciola gigantica

Synonyms

Synonyms Comment Organism
GST1
-
 Fasciola gigantica
mu-class glutathione S-transferase1
-
 Fasciola gigantica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
87.87
-
 1-chloro-2,4-dinitrobenzene wild type enzyme, at pH 7.5, temperature not specified in the publication Fasciola gigantica
100.25
-
 1-chloro-2,4-dinitrobenzene mutant enzyme F136A, at pH 7.5, temperature not specified in the publication Fasciola gigantica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
218.3
-
 1-chloro-2,4-dinitrobenzene wild type enzyme, at pH 7.5, temperature not specified in the publication Fasciola gigantica
328.3
-
 1-chloro-2,4-dinitrobenzene mutant enzyme F136A, at pH 7.5, temperature not specified in the publication Fasciola gigantica