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Literature summary for 2.5.1.18 extracted from

  • Shao, Y.; Lv, Z.; Li, C.; Zhang, W.; Duan, X.; Qiu, Q.; Jin, C.; Zhao, X.
    Molecular cloning and functional characterization of theta class glutathione S-transferase from Apostichopus japonicus (2017), Fish Shellfish Immunol., 63, 31-39 .
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Apostichopus japonicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Apostichopus japonicus 5829
-

Organism

Organism UniProt Comment Textmining
Apostichopus japonicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Sefinose resin column chromatography Apostichopus japonicus

Source Tissue

Source Tissue Comment Organism Textmining
coelomocyte
-
 Apostichopus japonicus
-
intestine highest expression Apostichopus japonicus
-
muscle lowest expression Apostichopus japonicus
-
respiratory system
-
 Apostichopus japonicus
-
tentacle
-
 Apostichopus japonicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Apostichopus japonicus chloride + 2,4-dinitrophenyl-glutathione
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Apostichopus japonicus
? x * 26560, calculated from amino acid sequence Apostichopus japonicus

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
 Apostichopus japonicus
GST-theta
-
 Apostichopus japonicus

pI Value

Organism Comment pI Value Maximum pI Value
Apostichopus japonicus calculated from amino acid sequence
-
 8.4

Expression

Organism Comment Expression
Apostichopus japonicus Vibrio splendidus challenge in vivo and lipopolysaccharide stimulation in vitro can both significantly up-regulate the mRNA expression of isoform GST-theta when compared with control group up

General Information

General Information Comment Organism
physiological function recombinant isoform GST-theta protein can markedly improve bacterial growth under cumene hydroperoxide exposure. More importantly, the recombinant isoform can effectively prevent primary coelomocytes apoptosis after lipopolysacchride exposure. Isoform GST-theta plays significantly roles in the modulation of immune response and protect cells from pathogens infection in Apostichopus japonicus Apostichopus japonicus