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Literature summary for 2.5.1.18 extracted from

  • Perperopoulou, F.; Ataya, F.S.; Fouad, D.; Malik, A.; Saeed, H.M.; Labrou, N.E.
    Biochemical characterization of the detoxifying enzyme glutathione transferase P1-1 from the camel Camelus dromedarius (2016), Cell Biochem. Biophys., 74, 459-472 .
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21(DE3) cells Camelus dromedarius

Inhibitors

Inhibitors Comment Organism Structure
alachlor about 90% residual activity at 0.1 mM Camelus dromedarius
alpha-endosulfan about 55% residual activity at 0.1 mM Camelus dromedarius
Atrazine about 95% residual activity at 0.1 mM Camelus dromedarius
beta-endosulfan about 90% residual activity at 0.1 mM Camelus dromedarius
carbendazim about 80% residual activity at 0.1 mM Camelus dromedarius
clothianidin about 85% residual activity at 0.1 mM Camelus dromedarius
desmedipham about 62% residual activity at 0.1 mM Camelus dromedarius
difheberan about 47% residual activity at 0.1 mM Camelus dromedarius
Diflubenzuron about 45% residual activity at 0.1 mM Camelus dromedarius
endosulfan about 73% residual activity at 0.1 mM Camelus dromedarius
epoxiconazole about 40% residual activity at 0.1 mM, complete inhibition at 0.2 mM Camelus dromedarius
fenamidone about 78% residual activity at 0.1 mM Camelus dromedarius
fenhexamid about 85% residual activity at 0.1 mM Camelus dromedarius
fludioxonil about 81% residual activity at 0.1 mM Camelus dromedarius
fluopyram about 82% residual activity at 0.1 mM Camelus dromedarius
fluoxastrobin about 75% residual activity at 0.1 mM Camelus dromedarius
imidacloprid about 92% residual activity at 0.1 mM Camelus dromedarius
iprodione about 60% residual activity at 0.1 mM Camelus dromedarius
kresoxim-methyl about 87% residual activity at 0.1 mM Camelus dromedarius
metalaxyl about 61% residual activity at 0.1 mM Camelus dromedarius
additional information not inhibited by spiroteramat, metribuzin and boscalid Camelus dromedarius
permethrin about 45% residual activity at 0.1 mM Camelus dromedarius
phenmedipham about 63% residual activity at 0.1 mM Camelus dromedarius
picoxystrobin about 58% residual activity at 0.1 mM Camelus dromedarius
piroxamine about 92% residual activity at 0.1 mM Camelus dromedarius
prothioconazol about 85% residual activity at 0.1 mM Camelus dromedarius
pyraclostrobin about 70% residual activity at 0.1 mM Camelus dromedarius
pyrimethanil about 80% residual activity at 0.1 mM Camelus dromedarius
sodium oxide about 50% residual activity at 0.1 mM Camelus dromedarius
teflubenzuron about 62% residual activity at 0.1 mM Camelus dromedarius
terbuthylazine about 97% residual activity at 0.1 mM Camelus dromedarius
tolclofos-methyl about 55% residual activity at 0.1 mM Camelus dromedarius
triadimenol about 75% residual activity at 0.1 mM Camelus dromedarius
trifloxystrobin about 65% residual activity at 0.1 mM Camelus dromedarius
triflumuron about 70% residual activity at 0.1 mM Camelus dromedarius
vinclozolin about 73% residual activity at 0.1 mM Camelus dromedarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
 phenethyl isothiocyanate at pH 7.0, temperature not specified in the publication Camelus dromedarius
0.12
-
 glutathione with cumene hydroperoxide as cosubstrate, at pH 7.0, temperature not specified in the publication Camelus dromedarius
0.25
-
 glutathione with phenethyl isothiocyanate as cosubstrate, at pH 7.0, temperature not specified in the publication Camelus dromedarius
0.45
-
 glutathione with 1-chloro-2,4-dinitrobenzene as cosubstrate, at pH 7.0, temperature not specified in the publication Camelus dromedarius
4.5
-
 1-chloro-2,4-dinitrobenzene at pH 7.0, temperature not specified in the publication Camelus dromedarius
14.3
-
 cumene hydroperoxide at pH 7.0, temperature not specified in the publication Camelus dromedarius

Organism

Organism UniProt Comment Textmining
Camelus dromedarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and GSH-Sepharose column chromatography Camelus dromedarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene 100% activity Camelus dromedarius chloride + 2,4-dinitrophenyl-glutathione
-
 ?
glutathione + 4-chloro-7-nitrobenzofurazan 8.2% activity compared to 1-chloro-2,4-dinitrobenzene Camelus dromedarius ?
-
 ?
glutathione + 4-nitrobenzyl chloride 30.5% activity compared to 1-chloro-2,4-dinitrobenzene Camelus dromedarius chloride + 4-nitrobenzyl-glutathione
-
 ?
glutathione + allyl isothiocyanate 58.9% activity compared to 1-chloro-2,4-dinitrobenzene Camelus dromedarius ?
-
 ?
glutathione + cumene hydroperoxide 56.3% activity compared to 1-chloro-2,4-dinitrobenzene Camelus dromedarius ?
-
 ?
glutathione + dehydroascorbate 43.6% activity compared to 1-chloro-2,4-dinitrobenzene Camelus dromedarius ?
-
 ?
glutathione + fluorodifen 1.2% activity compared to 1-chloro-2,4-dinitrobenzene Camelus dromedarius ?
-
 ?
glutathione + phenethyl isothiocyanate 129.7% activity compared to 1-chloro-2,4-dinitrobenzene Camelus dromedarius ?
-
 ?
glutathione + sulfanilamide 81.8% activity compared to 1-chloro-2,4-dinitrobenzene Camelus dromedarius ?
-
 ?
glutathione + trans-2-nonenal 5.9% activity compared to 1-chloro-2,4-dinitrobenzene Camelus dromedarius ?
-
 ?
glutathione + trans-4-phenyl-3-buten-2-one 1.2% activity compared to 1-chloro-2,4-dinitrobenzene Camelus dromedarius ?
-
 ?
additional information no activity with bromosulfophthalein, 2,3-dichloro-(4-[2-methylene-butyryl]phenoxy)acetic acid (ethacrynic acid), 2-hydroxyethyl disulfide (2,2-dithiodiethanol), tert-butyl hydroperoxide, and benzoyl peroxide Camelus dromedarius ?
-
-

Subunits

Subunits Comment Organism
? x * about 26000, SDS-PAGE Camelus dromedarius

Synonyms

Synonyms Comment Organism
GSTP1-1 isoenzyme Camelus dromedarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 39.4 the enzyme activity remains stable after 5 min at 0-37°C. The melting temperature is 39.4°C Camelus dromedarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 phenethyl isothiocyanate at pH 7.0, temperature not specified in the publication Camelus dromedarius
3
-
 glutathione with phenethyl isothiocyanate as cosubstrate, at pH 7.0, temperature not specified in the publication Camelus dromedarius
4.62
-
 1-chloro-2,4-dinitrobenzene at pH 7.0, temperature not specified in the publication Camelus dromedarius
4.62
-
 glutathione with 1-chloro-2,4-dinitrobenzene as cosubstrate, at pH 7.0, temperature not specified in the publication Camelus dromedarius
9.03
-
 cumene hydroperoxide at pH 7.0, temperature not specified in the publication Camelus dromedarius
9.03
-
 glutathione with cumene hydroperoxide as cosubstrate, at pH 7.0, temperature not specified in the publication Camelus dromedarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Camelus dromedarius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.63
-
 cumene hydroperoxide at pH 7.0, temperature not specified in the publication Camelus dromedarius
1.03
-
 1-chloro-2,4-dinitrobenzene at pH 7.0, temperature not specified in the publication Camelus dromedarius
64.1
-
 phenethyl isothiocyanate at pH 7.0, temperature not specified in the publication Camelus dromedarius