Protein Variants | Comment | Organism |
---|---|---|
I104V | the mutant shows about 6fold increased activity compared to the wild type enzyme | Homo sapiens |
I104V/A113V | the mutant shows about 3fold increased activity compared to the wild type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
gamma-Glu-Cys(Acm)-Gly | 85.2% remaining activity at 0.0003 mM | Homo sapiens | |
gamma-Glu-Cys(t-buthio)-Gly | 85.6% remaining activity at 0.0003 mM | Homo sapiens | |
gamma-Glu-Met-Gly | 79.4% remaining activity at 0.0003 mM | Homo sapiens | |
gamma-Glu-Thi-Gly | 67.4% remaining activity at 0.0003 mM | Homo sapiens | |
Gly-Cys-Hyp | 87.4% remaining activity at 0.0003 mM | Homo sapiens | |
Hyp-Cys-Ala | 98.7% remaining activity at 0.0003 mM | Homo sapiens | |
additional information | not inhibited by acetyl-gamma-Glu-Cys-Gly, gamma-Glu-Cys-Aib, gamma-Glu-Cys-a-t-butyl-Gly, Sar-Cys(Trt)-Gly, Tic-Cys-Ala, and gamma-Glu-NMeCys-Gly | Homo sapiens | |
pGlu-Cys(Trt)-Gly | 82.3% remaining activity at 0.0003 mM | Homo sapiens | |
Tic-Cys(Acm)-Gly | 68.5% remaining activity at 0.0003 mM | Homo sapiens | |
Tic-Cys-Aib | 80.1% remaining activity at 0.0003 mM | Homo sapiens | |
Tic-Cys-Sar | 53% remaining activity at 0.0003 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
gamma-Glu-Cys-Sar | mutant enzyme I104V/A113V, at pH 7.4 and 25°C | Homo sapiens | |
0.033 | - |
gamma-Glu-Cys-Sar | mutant enzyme I104V, at pH 7.4 and 25°C | Homo sapiens | |
0.074 | - |
glutathione | mutant enzyme I104V, at pH 7.4 and 25°C | Homo sapiens | |
0.15 | - |
glutathione | mutant enzyme I104V/A113V, at pH 7.4 and 25°C | Homo sapiens | |
0.3 | - |
glutathione | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
1.25 | - |
Tic-Cys-Gly | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
1.48 | - |
gamma-Glu-Cys-Aib | mutant enzyme I104V/A113V, at pH 7.4 and 25°C | Homo sapiens | |
2.24 | - |
acetyl-gamma-Glu-Cys-Gly | mutant enzyme I104V/A113V, at pH 7.4 and 25°C | Homo sapiens | |
3.18 | - |
gamma-Glu-Cys-Sar | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
3.22 | - |
Hyp-Cys-Gly | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09211 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-gamma-Glu-Cys-Gly + 1-chloro-2,4-dinitrobenzene | substrate for mutant enzyme I104V/A113V | Homo sapiens | ? | - |
? | |
gamma-Glu-Cys-Aib + 1-chloro-2,4-dinitrobenzene | substrate for mutant enzyme I104V/A113V | Homo sapiens | ? | - |
? | |
gamma-Glu-Cys-Nle + 1-chloro-2,4-dinitrobenzene | 26.4% activity compared to glutathione | Homo sapiens | ? | - |
? | |
gamma-Glu-Cys-Nva + 1-chloro-2,4-dinitrobenzene | 16% activity compared to glutathione | Homo sapiens | ? | - |
? | |
gamma-Glu-Cys-Sar + 1-chloro-2,4-dinitrobenzene | 17% activity compared to glutathione | Homo sapiens | ? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | 100% activity | Homo sapiens | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
Hyp-Cys-Gly + 1-chloro-2,4-dinitrobenzene | 39.5% activity compared to glutathione | Homo sapiens | ? | - |
? | |
additional information | the wild type enzyme shows no activity with acetyl-gamma-Glu-Cys-Gly, gamma-Glu-NMeCys-Gly, gamma-Glu-Cys-Aib, gamma-Glu-Cys-a-t-butyl-Gly, Tic-Cys-Ala, Hyp-Cys-Ala, Gly-Cys-Hyp, Tic-Cys-Aib, Tic-Cys(Acm)-Gly, and Tic-Cys-Sar | Homo sapiens | ? | - |
- |
|
NMe-Glu-Cys-Gly + 1-chloro-2,4-dinitrobenzene | 3.9% activity compared to glutathione | Homo sapiens | ? | - |
? | |
pGlu-Cys-Gly + 1-chloro-2,4-dinitrobenzene | 11.5% activity compared to glutathione | Homo sapiens | ? | - |
? | |
Pro-Cys-Gly + 1-chloro-2,4-dinitrobenzene | 21.6% activity compared to glutathione | Homo sapiens | ? | - |
? | |
Sar-Cys-Gly + 1-chloro-2,4-dinitrobenzene | 15.8% activity compared to glutathione | Homo sapiens | ? | - |
? | |
Tic-Cys-Gly + 1-chloro-2,4-dinitrobenzene | 41.3% activity compared to glutathione | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione transferase P1-1 | - |
Homo sapiens |
GSTP1-1 | isoform | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
Tic-Cys-Gly | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
0.39 | - |
gamma-Glu-Cys-Sar | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
0.53 | - |
gamma-Glu-Cys-Sar | mutant enzyme I104V, at pH 7.4 and 25°C | Homo sapiens | |
0.56 | - |
gamma-Glu-Cys-Sar | mutant enzyme I104V/A113V, at pH 7.4 and 25°C | Homo sapiens | |
1.03 | - |
acetyl-gamma-Glu-Cys-Gly | mutant enzyme I104V/A113V, at pH 7.4 and 25°C | Homo sapiens | |
1.04 | - |
Hyp-Cys-Gly | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
1.7 | - |
glutathione | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
1.91 | - |
gamma-Glu-Cys-Aib | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
2.29 | - |
glutathione | mutant enzyme I104V/A113V, at pH 7.4 and 25°C | Homo sapiens | |
2.52 | - |
glutathione | mutant enzyme I104V, at pH 7.4 and 25°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
gamma-Glu-Cys-Sar | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
0.25 | - |
Tic-Cys-Gly | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
0.32 | - |
Hyp-Cys-Gly | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
0.46 | - |
acetyl-gamma-Glu-Cys-Gly | mutant enzyme I104V/A113V, at pH 7.4 and 25°C | Homo sapiens | |
1.29 | - |
gamma-Glu-Cys-Aib | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
5.67 | - |
glutathione | wild type enzyme, at pH 7.4 and 25°C | Homo sapiens | |
15.27 | - |
glutathione | mutant enzyme I104V/A113V, at pH 7.4 and 25°C | Homo sapiens | |
16.07 | - |
gamma-Glu-Cys-Sar | mutant enzyme I104V, at pH 7.4 and 25°C | Homo sapiens | |
34 | - |
glutathione | mutant enzyme I104V, at pH 7.4 and 25°C | Homo sapiens | |
39.68 | - |
gamma-Glu-Cys-Sar | mutant enzyme I104V/A113V, at pH 7.4 and 25°C | Homo sapiens |