Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
dehydroascorbate | isoform GstO2B, at pH 7.6 and 30°C | Drosophila melanogaster | |
1.03 | - |
dehydroascorbate | isoform GstO2A, at pH 7.6 and 30°C | Drosophila melanogaster | |
4.23 | - |
glutathione | isoform GstO2A, at pH 7.6 and 30°C | Drosophila melanogaster | |
4.33 | - |
glutathione | isoform GstO2B, at pH 7.6 and 30°C | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Drosophila melanogaster | 5737 | - |
mitochondrion | - |
Drosophila melanogaster | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28700 | - |
- |
Drosophila melanogaster |
29100 | - |
- |
Drosophila melanogaster |
30000 | - |
2 * 30000, SDS-PAGE | Drosophila melanogaster |
60000 | - |
gel filtration | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9VSL4 | isoform GstO2B | - |
Drosophila melanogaster | Q9VSL5 | isoform GstO2A | - |
Purification (Comment) | Organism |
---|---|
Ni2+-affinity resin column chromatography | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dehydroascorbate + glutathione | - |
Drosophila melanogaster | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 30000, SDS-PAGE | Drosophila melanogaster |
homodimer | 2 * 28700, isoform GstO2B, calculated from amino acid sequence | Drosophila melanogaster |
homodimer | 2 * 29100, isoform GstO2A, calculated from amino acid sequence | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase omega 2 | - |
Drosophila melanogaster |
GstO2A | isoform | Drosophila melanogaster |
GstO2B | isoform | Drosophila melanogaster |
omega-class glutathione S-transferase 2 | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Drosophila melanogaster |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
673.7 | - |
dehydroascorbate | isoform GstO2A, at pH 7.6 and 30°C | Drosophila melanogaster | |
692.8 | - |
glutathione | isoform GstO2A, at pH 7.6 and 30°C | Drosophila melanogaster | |
905.8 | - |
glutathione | isoform GstO2B, at pH 7.6 and 30°C | Drosophila melanogaster | |
925.2 | - |
dehydroascorbate | isoform GstO2B, at pH 7.6 and 30°C | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Drosophila melanogaster |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
164 | - |
glutathione | isoform GstO2A, at pH 7.6 and 30°C | Drosophila melanogaster | |
209 | - |
glutathione | isoform GstO2B, at pH 7.6 and 30°C | Drosophila melanogaster | |
654 | - |
dehydroascorbate | isoform GstO2A, at pH 7.6 and 30°C | Drosophila melanogaster | |
2370 | - |
dehydroascorbate | isoform GstO2B, at pH 7.6 and 30°C | Drosophila melanogaster |