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Literature summary for 2.5.1.18 extracted from

  • Yang, X.Q.; Zhang, Y.L.
    Characterization of glutathione S-transferases from Sus scrofa, Cydia pomonella and Triticum aestivum: their responses to cantharidin (2015), Enzyme Microb. Technol., 69, 1-9.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cantharidin cantharidin significantly stimulates isoform GSTf3 activity Triticum aestivum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Sus scrofa
expressed in Escherichia coli BL21(DE3) cells Triticum aestivum
expressed in Escherichia coli BL21(DE3) cells Cydia pomonella

Inhibitors

Inhibitors Comment Organism Structure
cantharidic acid
-
Cydia pomonella
cantharidic acid
-
Sus scrofa
cantharidic acid
-
Triticum aestivum
cantharidin
-
Cydia pomonella
additional information cantharidin has no effect on isoform SGSTalpha1 activity Sus scrofa
S-hexylglutathione
-
Cydia pomonella
S-hexylglutathione
-
Sus scrofa
S-hexylglutathione
-
Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.091
-
1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Sus scrofa
0.2
-
1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Triticum aestivum
0.27
-
1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Cydia pomonella
0.36
-
glutathione at pH 7.5 and 30°C Triticum aestivum
0.79
-
glutathione at pH 7.5 and 30°C Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24190
-
-
Cydia pomonella
24490
-
-
Triticum aestivum
25280
-
-
Sus scrofa

Organism

Organism UniProt Comment Textmining
Cydia pomonella
-
-
-
Sus scrofa
-
-
-
Triticum aestivum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA agarose column chromatography Sus scrofa
Ni2+-NTA agarose column chromatography Triticum aestivum
Ni2+-NTA agarose column chromatography Cydia pomonella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
Sus scrofa S-(2,4-dinitrophenyl)glutathione + HCl
-
?
1-chloro-2,4-dinitrobenzene + glutathione
-
Triticum aestivum S-(2,4-dinitrophenyl)glutathione + HCl
-
?
1-chloro-2,4-dinitrobenzene + glutathione
-
Cydia pomonella S-(2,4-dinitrophenyl)glutathione + HCl
-
?

Subunits

Subunits Comment Organism
? x * 24190, calculated from amino acid sequence Cydia pomonella
? x * 24490, calculated from amino acid sequence Triticum aestivum
? x * 25280, calculated from amino acid sequence Sus scrofa

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
Sus scrofa
glutathione S-transferase
-
Triticum aestivum
glutathione S-transferase
-
Cydia pomonella
GSTalpha1
-
Sus scrofa
GSTD1
-
Cydia pomonella
GSTF3
-
Triticum aestivum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Sus scrofa
30
-
-
Triticum aestivum
30
-
-
Cydia pomonella

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 more than 50% activity between 20 and 40°C Sus scrofa
20 40 more than 50% activity between 20 and 40°C Triticum aestivum
20 40 more than 50% activity between 20 and 40°C Cydia pomonella

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.9
-
1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Triticum aestivum
16.4
-
glutathione at pH 7.5 and 30°C Triticum aestivum
99
-
1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Sus scrofa
141.7
-
glutathione at pH 7.5 and 30°C Sus scrofa
610
-
1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Cydia pomonella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 at 30°C Sus scrofa
7 7.5 at 30°C Triticum aestivum
7 7.5 at 30°C Cydia pomonella

pH Range

pH Minimum pH Maximum Comment Organism
6 8 more than 50% activity between pH 6.0 and 8.0 Sus scrofa
6 8 more than 50% activity between pH 6.0 and 8.0 Triticum aestivum
6 8 more than 50% activity between pH 6.0 and 8.0 Cydia pomonella

pI Value

Organism Comment pI Value Maximum pI Value
Triticum aestivum calculated from amino acid sequence
-
5.3
Cydia pomonella calculated from amino acid sequence
-
6.8
Sus scrofa calculated from amino acid sequence
-
8.9

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
55
-
1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Triticum aestivum
91
-
glutathione at pH 7.5 and 30°C Triticum aestivum
109
-
1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Sus scrofa
178
-
glutathione at pH 7.5 and 30°C Sus scrofa
2283
-
1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Cydia pomonella