Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cantharidin | cantharidin significantly stimulates isoform GSTf3 activity | Triticum aestivum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Sus scrofa |
expressed in Escherichia coli BL21(DE3) cells | Triticum aestivum |
expressed in Escherichia coli BL21(DE3) cells | Cydia pomonella |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cantharidic acid | - |
Cydia pomonella | |
cantharidic acid | - |
Sus scrofa | |
cantharidic acid | - |
Triticum aestivum | |
cantharidin | - |
Cydia pomonella | |
additional information | cantharidin has no effect on isoform SGSTalpha1 activity | Sus scrofa | |
S-hexylglutathione | - |
Cydia pomonella | |
S-hexylglutathione | - |
Sus scrofa | |
S-hexylglutathione | - |
Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.091 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.5 and 30°C | Sus scrofa | |
0.2 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.5 and 30°C | Triticum aestivum | |
0.27 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.5 and 30°C | Cydia pomonella | |
0.36 | - |
glutathione | at pH 7.5 and 30°C | Triticum aestivum | |
0.79 | - |
glutathione | at pH 7.5 and 30°C | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24190 | - |
- |
Cydia pomonella |
24490 | - |
- |
Triticum aestivum |
25280 | - |
- |
Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cydia pomonella | - |
- |
- |
Sus scrofa | - |
- |
- |
Triticum aestivum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose column chromatography | Sus scrofa |
Ni2+-NTA agarose column chromatography | Triticum aestivum |
Ni2+-NTA agarose column chromatography | Cydia pomonella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + glutathione | - |
Sus scrofa | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
1-chloro-2,4-dinitrobenzene + glutathione | - |
Triticum aestivum | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
1-chloro-2,4-dinitrobenzene + glutathione | - |
Cydia pomonella | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24190, calculated from amino acid sequence | Cydia pomonella |
? | x * 24490, calculated from amino acid sequence | Triticum aestivum |
? | x * 25280, calculated from amino acid sequence | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Sus scrofa |
glutathione S-transferase | - |
Triticum aestivum |
glutathione S-transferase | - |
Cydia pomonella |
GSTalpha1 | - |
Sus scrofa |
GSTD1 | - |
Cydia pomonella |
GSTF3 | - |
Triticum aestivum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Sus scrofa |
30 | - |
- |
Triticum aestivum |
30 | - |
- |
Cydia pomonella |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | more than 50% activity between 20 and 40°C | Sus scrofa |
20 | 40 | more than 50% activity between 20 and 40°C | Triticum aestivum |
20 | 40 | more than 50% activity between 20 and 40°C | Cydia pomonella |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.9 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.5 and 30°C | Triticum aestivum | |
16.4 | - |
glutathione | at pH 7.5 and 30°C | Triticum aestivum | |
99 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.5 and 30°C | Sus scrofa | |
141.7 | - |
glutathione | at pH 7.5 and 30°C | Sus scrofa | |
610 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.5 and 30°C | Cydia pomonella |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | at 30°C | Sus scrofa |
7 | 7.5 | at 30°C | Triticum aestivum |
7 | 7.5 | at 30°C | Cydia pomonella |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | more than 50% activity between pH 6.0 and 8.0 | Sus scrofa |
6 | 8 | more than 50% activity between pH 6.0 and 8.0 | Triticum aestivum |
6 | 8 | more than 50% activity between pH 6.0 and 8.0 | Cydia pomonella |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Triticum aestivum | calculated from amino acid sequence | - |
5.3 |
Cydia pomonella | calculated from amino acid sequence | - |
6.8 |
Sus scrofa | calculated from amino acid sequence | - |
8.9 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
55 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.5 and 30°C | Triticum aestivum | |
91 | - |
glutathione | at pH 7.5 and 30°C | Triticum aestivum | |
109 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.5 and 30°C | Sus scrofa | |
178 | - |
glutathione | at pH 7.5 and 30°C | Sus scrofa | |
2283 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.5 and 30°C | Cydia pomonella |