Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) pLysS cells | Nilaparvata lugens |
Crystallization (Comment) | Organism |
---|---|
in complex with glutathione, sitting drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.0) containing 1 M LiCl and 30% (w/v) PEG-6000 | Nilaparvata lugens |
Protein Variants | Comment | Organism |
---|---|---|
E66A | the mutant shows reduced activity compared to the wild type enzyme | Nilaparvata lugens |
F119A | the mutant shows reduced activity compared to the wild type enzyme | Nilaparvata lugens |
H52A | the mutant shows reduced activity compared to the wild type enzyme | Nilaparvata lugens |
R68A | the mutant shows reduced activity compared to the wild type enzyme | Nilaparvata lugens |
S11A | the mutant shows reduced activity compared to the wild type enzyme | Nilaparvata lugens |
S67A | the mutant shows reduced activity compared to the wild type enzyme | Nilaparvata lugens |
V54A | the mutant shows reduced activity compared to the wild type enzyme | Nilaparvata lugens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
glutathione | wild type enzyme, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.19 | - |
glutathione | mutant enzyme S67A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.26 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme S11A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.41 | - |
glutathione | mutant enzyme F119A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.42 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F119A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.46 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme V54A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.73 | - |
glutathione | mutant enzyme H52A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.74 | - |
glutathione | mutant enzyme S11A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.84 | - |
1-chloro-2,4-dinitrobenzene | wild type enzyme, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.93 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme R68A, at pH 8.0 and 30°C | Nilaparvata lugens | |
1 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme H52A, at pH 8.0 and 30°C | Nilaparvata lugens | |
1 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme S67A, at pH 8.0 and 30°C | Nilaparvata lugens | |
1.2 | - |
glutathione | mutant enzyme V54A, at pH 8.0 and 30°C | Nilaparvata lugens | |
1.7 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme E66A, at pH 8.0 and 30°C | Nilaparvata lugens | |
3.3 | - |
glutathione | mutant enzyme R68A, at pH 8.0 and 30°C | Nilaparvata lugens | |
3.4 | - |
glutathione | mutant enzyme E66A, at pH 8.0 and 30°C | Nilaparvata lugens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nilaparvata lugens | Q8N0B3 | strain Izumo | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography and Superdex 200 gel filtration | Nilaparvata lugens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + glutathione | - |
Nilaparvata lugens | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
additional information | no activity with 4-hydroxynonenal, ethacrynic acid, 4-nitrobenzyl chloride, 1,2-epoxy-3-(4-nitrophenoxy)-propane, 4-nitrophenethyl bromide, and 4-nitrophenyl acetate | Nilaparvata lugens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Nilaparvata lugens |
Synonyms | Comment | Organism |
---|---|---|
Delta-class glutathione transferase | - |
Nilaparvata lugens |
GSTD | - |
Nilaparvata lugens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme is stable at temperatures below 50°C | Nilaparvata lugens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
glutathione | mutant enzyme S11A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.27 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme S11A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.33 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F119A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.52 | - |
glutathione | mutant enzyme F119A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.58 | - |
glutathione | mutant enzyme H52A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.78 | - |
glutathione | mutant enzyme V54A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.85 | - |
glutathione | mutant enzyme E66A, at pH 8.0 and 30°C | Nilaparvata lugens | |
1.07 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme V54A, at pH 8.0 and 30°C | Nilaparvata lugens | |
1.27 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme H52A, at pH 8.0 and 30°C | Nilaparvata lugens | |
1.62 | - |
glutathione | wild type enzyme, at pH 8.0 and 30°C | Nilaparvata lugens | |
1.83 | - |
glutathione | mutant enzyme S67A, at pH 8.0 and 30°C | Nilaparvata lugens | |
2.67 | - |
1-chloro-2,4-dinitrobenzene | wild type enzyme, at pH 8.0 and 30°C | Nilaparvata lugens | |
2.67 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme E66A, at pH 8.0 and 30°C | Nilaparvata lugens | |
2.67 | - |
glutathione | mutant enzyme R68A, at pH 8.0 and 30°C | Nilaparvata lugens | |
3 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme S67A, at pH 8.0 and 30°C | Nilaparvata lugens | |
3.17 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme R68A, at pH 8.0 and 30°C | Nilaparvata lugens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 12 | the enzyme retains more than 80% of its original activity when shifted from pH 6.0 to 12.0 | Nilaparvata lugens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000217 | - |
glutathione | mutant enzyme S11A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.000025 | - |
glutathione | mutant enzyme E66A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.000065 | - |
glutathione | mutant enzyme V54A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.00008 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F119A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.00008 | - |
glutathione | mutant enzyme H52A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.0000817 | - |
glutathione | mutant enzyme R68A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.000103 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme S11A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.000127 | - |
glutathione | mutant enzyme F119A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.000127 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme H52A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.000153 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme E66A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.0023 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme V54A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.003 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme S67A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.0032 | - |
1-chloro-2,4-dinitrobenzene | wild type enzyme, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.0033 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme R68A, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.009 | - |
glutathione | wild type enzyme, at pH 8.0 and 30°C | Nilaparvata lugens | |
0.0098 | - |
glutathione | mutant enzyme S67A, at pH 8.0 and 30°C | Nilaparvata lugens |