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Literature summary for 2.5.1.18 extracted from

  • Zhan, B.; Perally, S.; Brophy, P.M.; Xue, J.; Goud, G.; Liu, S.; Deumic, V.; de Oliveira, L.M.; Bethony, J.; Bottazzi, M.E.; Jiang, D.; Gillespie, P.; Xiao, S.H.; Gupta, R.; Loukas, A.; Ranjit, N.; Lustigman, S.; Oksov, Y.; Hotez, P.
    Molecular cloning, biochemical characterization, and partial protective immunity of the heme-binding glutathione S-transferases from the human hookworm Necator americanus (2010), Infect. Immun., 78, 1552-1563.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine the GST isozymes are useful as vaccine against hookworm infection Necator americanus

Cloned(Commentary)

Cloned (Comment) Organism
genes Na-gst-1, Na-gst-2, and Na-gst-3, encoding isozymes GST-1, GST-2, and GST-3, phylogenetic analysis, recombinant expression in Pichia pastoris, the recombinant isozymes all exhibit low lipid peroxidase and glutathione-conjugating enzymatic activities but high heme-binding capacities Necator americanus

Inhibitors

Inhibitors Comment Organism Structure
Hematin binding by isozymes with high affinity Necator americanus

Organism

Organism UniProt Comment Textmining
Necator americanus D3U1A5 genes Na-gst-1, Na-gst-2, and Na-gst-3 encoding isozymes GST-1, GST-2, and GST-3
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
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Necator americanus S-(2,4-dinitrophenyl)glutathione + HCl
-
?
additional information recombinant isozymes GST-1, GST-2, and GST-3, expressed in Pichia pastoris, all exhibit low lipid peroxidase and glutathione-conjugating enzymatic activities but high heme-binding capacities Necator americanus ?
-
?

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
Necator americanus
GST
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Necator americanus
More the Necator americanus GST isozymes belong to a nematode-specific nu-class GST family Necator americanus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Necator americanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Necator americanus

General Information

General Information Comment Organism
physiological function the isozymes may be involved in the detoxification and/or transport of heme Necator americanus