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Literature summary for 2.5.1.18 extracted from

  • Yamamoto, K.; Nagaoka, S.; Banno, Y.; Aso, Y.
    Biochemical properties of an omega-class glutathione S-transferase of the silkmoth, Bombyx mori (2009), Comp. Biochem. Physiol. C, 149, 461-467.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozyme GSTO, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional overexpression in Escherichia coli as soluble protein Bombyx mori
sequence comparisons and phylogenetic analysis Bombyx mori

Inhibitors

Inhibitors Comment Organism Structure
deltamethrin 41% inhibition at 1 mM; 54% inhibition at 1 mM Bombyx mori
fenitrothion 50% inhibition at 1 mM; 56% inhibition at 1 mM Bombyx mori
additional information the enzyme is completely resistant against insecticides fenitrothion, deltamethrin, and permethrin, in vivo toxicity, overview Bombyx mori
permethrin 24% inhibition at 1 mM; 33% inhibition at 1 mM Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Bombyx mori
0.006
-
4-hydroxynonenal GSTO Bombyx mori
0.14
-
4-hydroxynonenal GSTD Bombyx mori
0.48
-
1-chloro-2,4-dinitrobenzene GSTD Bombyx mori
0.67
-
1-chloro-2,4-dinitrobenzene GSTO Bombyx mori
1.6
-
1-chloro-2,4-dinitrobenzene GSTS Bombyx mori
2.1
-
4-hydroxynonenal GSTS Bombyx mori

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Bombyx mori 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23338
-
x * 23338, GSTO, sequence calculation Bombyx mori
24225
-
x * 24225, GSTO, sequence calculation Bombyx mori
29806
-
x * 29806, GSTO, sequence calculation Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori A8R5V3 omega-GST
-
Bombyx mori Q5CCJ4 sigma-GST
-
Bombyx mori Q60GK5 delta-GST
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme GSTO from Escherichia coli to homogeneity by ammonium sulfate fractionation, anion exchange chromatography and gel filtration Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
fat body
-
Bombyx mori
-
larva fifth-instar larvae Bombyx mori
-
midgut
-
Bombyx mori
-
silk gland low expression level Bombyx mori
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
Bombyx mori S-(2,4-dinitrophenyl)glutathione + HCl
-
?
4-hydroxynonenal + glutathione
-
Bombyx mori ?
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
-
Bombyx mori S-(2,4-dinitrophenyl)glutathione + HCl
-
?
glutathione + 4-hydroxynonenal a lipid peroxidation product Bombyx mori ?
-
?
glutathione + 4-nitrophenyl acetate
-
Bombyx mori S-(4-nitrophenyl)glutathione + HCl
-
?
glutathione + ethacrynic acid
-
Bombyx mori ?
-
?
additional information the enzyme shows high affinity for organophosphorus insecticide Bombyx mori ?
-
?

Subunits

Subunits Comment Organism
? x * 23338, GSTO, sequence calculation Bombyx mori
? x * 24225, GSTO, sequence calculation Bombyx mori
? x * 29806, GSTO, sequence calculation Bombyx mori

Synonyms

Synonyms Comment Organism
delta-class glutathione S-transferase
-
Bombyx mori
glutathione S-transferase
-
Bombyx mori
GSTD
-
Bombyx mori
GSTO
-
Bombyx mori
GSTS
-
Bombyx mori
omega-class glutathione S-transferase
-
Bombyx mori
sigma-class glutathione S-transferase
-
Bombyx mori
sigma-class GST
-
Bombyx mori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
purified GSTS, stable up to Bombyx mori
50
-
purified GSTD, stable up to Bombyx mori
50
-
purified GSTO, stable up to Bombyx mori
60
-
purified GSTO, 50% inhibitioin Bombyx mori
80
-
purified GSTO, inactivation Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 9 GSTD Bombyx mori
7 8 GSTO Bombyx mori
8
-
GSTS Bombyx mori

pH Range

pH Minimum pH Maximum Comment Organism
2 10 activity range, about 10% of maximal activity at pH 2.0 and pH 10.0 Bombyx mori

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 12 GSTO shows 75% of maximal activity at pH 2.0 and pH 12.0 after 24 h at 4°C Bombyx mori
4 11 GSTS Bombyx mori
5 11 GSTD Bombyx mori

pI Value

Organism Comment pI Value Maximum pI Value
Bombyx mori GSTS, sequence calculation
-
5.79
Bombyx mori GSTO, sequence calculation
-
6.01
Bombyx mori GSTD, sequence calculation
-
8.35

General Information

General Information Comment Organism
physiological function bmGSTO might be involved in the increase in level of insecticide resistance for lepidopteran insects Bombyx mori