Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain XL1-Blue | Mus musculus |
expression of His-tagged wild-type enzyme in Escherichia coli strain XL1-Blue | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
R234W | site-directed mutagenesis | Mus musculus |
W234R | site-directed mutagenesis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis, overview | Rattus norvegicus | |
additional information | - |
additional information | kinetic analysis of wild-type and mutant enzymes, overview | Mus musculus | |
additional information | - |
additional information | kinetic analysis of wild-type and mutant enzymes, overview | Homo sapiens | |
0.05 | - |
iodohexane | pH 8.0, 30°C, recombinant enzyme | Rattus norvegicus | |
0.058 | - |
iodohexane | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
0.059 | - |
1-iodohexane | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
0.083 | - |
iodobutane | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
0.085 | - |
iodomethane | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
0.36 | - |
iodobutane | pH 8.0, 30°C, recombinant enzyme | Rattus norvegicus | |
0.56 | - |
1-Iodobutane | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
0.91 | - |
1-iodomethane | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
1.89 | - |
iodomethane | pH 8.0, 30°C, recombinant enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P30711 | - |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain XL1-Blue by nickel affinity chromatography | Mus musculus |
recombinant His-tagged wild-type enzyme from Escherichia coli strain XL1-Blue by nickel affinity chromatography | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificities of recombinant His-tagged wild-type and mutant enzymes, overview | Mus musculus |
additional information | - |
substrate specificities of recombinant His-tagged wild-type and mutant enzymes, overview | Homo sapiens |
additional information | - |
substrate specificity of recombinant His-tagged enzyme, overview | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione | - |
Mus musculus | ? | - |
? | |
1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione | - |
Rattus norvegicus | ? | - |
? | |
1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione | - |
Homo sapiens | ? | - |
? | |
4-nitrobenzyl chloride + glutathione | - |
Mus musculus | ? | - |
? | |
4-nitrobenzyl chloride + glutathione | - |
Rattus norvegicus | ? | - |
? | |
4-nitrobenzyl chloride + glutathione | - |
Homo sapiens | ? | - |
? | |
4-nitrophenethyl bromide + glutathione | - |
Mus musculus | ? | - |
? | |
4-nitrophenethyl bromide + glutathione | - |
Rattus norvegicus | ? | - |
? | |
4-nitrophenethyl bromide + glutathione | - |
Homo sapiens | ? | - |
? | |
allyl isothiocyanate + glutathione | - |
Mus musculus | ? | - |
? | |
allyl isothiocyanate + glutathione | - |
Rattus norvegicus | ? | - |
? | |
allyl isothiocyanate + glutathione | - |
Homo sapiens | ? | - |
? | |
benzyl isothiocyanate + glutathione | - |
Mus musculus | ? | - |
? | |
benzyl isothiocyanate + glutathione | - |
Rattus norvegicus | ? | - |
? | |
benzyl isothiocyanate + glutathione | - |
Homo sapiens | ? | - |
? | |
cumene hydroperoxide + glutathione | - |
Mus musculus | ? | - |
? | |
cumene hydroperoxide + glutathione | - |
Rattus norvegicus | ? | - |
? | |
cumene hydroperoxide + glutathione | - |
Homo sapiens | ? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Mus musculus | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Rattus norvegicus | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Homo sapiens | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
glutathione + 1-iodobutane | - |
Mus musculus | iodide + butyl-glutathione | - |
? | |
glutathione + 1-iodohexane | - |
Mus musculus | iodide + hexyl-glutathione | - |
? | |
glutathione + 1-iodomethane | - |
Mus musculus | iodide + methylglutathione | - |
? | |
hydrogen peroxide + glutathione | - |
Mus musculus | ? | - |
? | |
hydrogen peroxide + glutathione | - |
Rattus norvegicus | ? | - |
? | |
hydrogen peroxide + glutathione | - |
Homo sapiens | ? | - |
? | |
iodobutane + glutathione | - |
Rattus norvegicus | ? | - |
? | |
iodohexane + glutathione | - |
Rattus norvegicus | ? | - |
? | |
iodomethane + glutathione | - |
Rattus norvegicus | ? | - |
? | |
additional information | substrate specificity, overview. A major structural determinant of substrate recognition is the H-site, which binds the electrophile in proximity to the nucleophilic sulfur of the second substrate glutathione. H-site residue 234 has a key role in governing the activity and substrate selectivity profile of GST T1-1 | Mus musculus | ? | - |
? | |
additional information | substrate specificity, overview. A major structural determinant of substrate recognition is the H-site, which binds the electrophile in proximity to the nucleophilic sulfur of the second substrate glutathione. H-site residue 234 has a key role in governing the activity and substrate selectivity profile of GST T1-1 | Rattus norvegicus | ? | - |
? | |
additional information | substrate specificity, overview. A major structural determinant of substrate recognition is the H-site, which binds the electrophile in proximity to the nucleophilic sulfur of the second substrate glutathione. H-site residue 234 has a key role in governing the activity and substrate selectivity profile of GST T1-1 | Homo sapiens | ? | - |
? | |
phenethyl isothiocyanate + glutathione | - |
Mus musculus | ? | - |
? | |
phenethyl isothiocyanate + glutathione | - |
Rattus norvegicus | ? | - |
? | |
phenethyl isothiocyanate + glutathione | - |
Homo sapiens | ? | - |
? | |
propyl isothiocyanate + glutathione | - |
Mus musculus | ? | - |
? | |
propyl isothiocyanate + glutathione | - |
Rattus norvegicus | ? | - |
? | |
propyl isothiocyanate + glutathione | - |
Homo sapiens | ? | - |
? | |
styrene oxide + glutathione | - |
Mus musculus | ? | - |
? | |
styrene oxide + glutathione | - |
Rattus norvegicus | ? | - |
? | |
styrene oxide + glutathione | - |
Homo sapiens | ? | - |
? | |
tertiary butyl hydroperoxide + glutathione | - |
Mus musculus | ? | - |
? | |
tertiary butyl hydroperoxide + glutathione | - |
Rattus norvegicus | ? | - |
? | |
tertiary butyl hydroperoxide + glutathione | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the substrate recognition H-site is formed by several segments of amino acid residues located in separate regions of the primary structure. The C-terminal helix of the protein serves as a lid over the active site, and contributes several residues to the H-site, molecular modeling, overview | Mus musculus |
More | the substrate recognition H-site is formed by several segments of amino acid residues located in separate regions of the primary structure. The C-terminal helix of the protein serves as a lid over the active site, and contributes several residues to the H-site, molecular modeling, overview | Rattus norvegicus |
More | the substrate recognition H-site is formed by several segments of amino acid residues located in separate regions of the primary structure. The C-terminal helix of the protein serves as a lid over the active site, and contributes several residues to the H-site, molecular modeling, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GST T1-1 | - |
Mus musculus |
GST T1-1 | - |
Rattus norvegicus |
GST T1-1 | - |
Homo sapiens |
theta class glutathione transferase T1-1 | - |
Mus musculus |
theta class glutathione transferase T1-1 | - |
Rattus norvegicus |
theta class glutathione transferase T1-1 | - |
Homo sapiens |
theta class GST | - |
Mus musculus |
theta class GST | - |
Rattus norvegicus |
theta class GST | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
30 | - |
assay at | Rattus norvegicus |
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
iodobutane | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
0.068 | - |
iodohexane | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
0.137 | - |
1-Iodobutane | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
0.215 | - |
iodobutane | pH 8.0, 30°C, recombinant enzyme | Rattus norvegicus | |
3.17 | - |
1-iodohexane | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
3.52 | - |
iodohexane | pH 8.0, 30°C, recombinant enzyme | Rattus norvegicus | |
9.16 | - |
iodomethane | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
238 | - |
1-iodomethane | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
349 | - |
iodomethane | pH 8.0, 30°C, recombinant enzyme | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | assay at, dependent on the substrate | Mus musculus |
6.5 | 8 | assay at, dependent on the substrate | Rattus norvegicus |
6.5 | 8 | assay at, dependent on the substrate | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.244 | - |
iodobutane | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
0.59 | - |
iodobutane | pH 8.0, 30°C, recombinant enzyme | Rattus norvegicus | |
0.701 | - |
iodobutane | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
1.17 | - |
iodohexane | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
54.1 | - |
iodohexane | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
69.8 | - |
iodohexane | pH 8.0, 30°C, recombinant enzyme | Rattus norvegicus | |
107 | - |
iodomethane | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
185 | - |
iodomethane | pH 8.0, 30°C, recombinant enzyme | Rattus norvegicus | |
263 | - |
iodomethane | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus |