Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.18 extracted from

  • Shokeer, A.; Mannervik, B.
    Residue 234 is a master switch of the alternative-substrate activity profile of human and rodent theta class glutathione transferase T1-1 (2010), Biochim. Biophys. Acta, 1800, 466-473.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain XL1-Blue Mus musculus
expression of His-tagged wild-type enzyme in Escherichia coli strain XL1-Blue Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
R234W site-directed mutagenesis Mus musculus
W234R site-directed mutagenesis Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic analysis, overview Rattus norvegicus
additional information
-
additional information kinetic analysis of wild-type and mutant enzymes, overview Mus musculus
additional information
-
additional information kinetic analysis of wild-type and mutant enzymes, overview Homo sapiens
0.05
-
iodohexane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
0.058
-
iodohexane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
0.059
-
1-iodohexane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
0.083
-
iodobutane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
0.085
-
iodomethane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
0.36
-
iodobutane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
0.56
-
1-Iodobutane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
0.91
-
1-iodomethane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
1.89
-
iodomethane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens P30711
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain XL1-Blue by nickel affinity chromatography Mus musculus
recombinant His-tagged wild-type enzyme from Escherichia coli strain XL1-Blue by nickel affinity chromatography Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
substrate specificities of recombinant His-tagged wild-type and mutant enzymes, overview Mus musculus
additional information
-
substrate specificities of recombinant His-tagged wild-type and mutant enzymes, overview Homo sapiens
additional information
-
substrate specificity of recombinant His-tagged enzyme, overview Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione
-
Mus musculus ?
-
?
1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione
-
Rattus norvegicus ?
-
?
1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione
-
Homo sapiens ?
-
?
4-nitrobenzyl chloride + glutathione
-
Mus musculus ?
-
?
4-nitrobenzyl chloride + glutathione
-
Rattus norvegicus ?
-
?
4-nitrobenzyl chloride + glutathione
-
Homo sapiens ?
-
?
4-nitrophenethyl bromide + glutathione
-
Mus musculus ?
-
?
4-nitrophenethyl bromide + glutathione
-
Rattus norvegicus ?
-
?
4-nitrophenethyl bromide + glutathione
-
Homo sapiens ?
-
?
allyl isothiocyanate + glutathione
-
Mus musculus ?
-
?
allyl isothiocyanate + glutathione
-
Rattus norvegicus ?
-
?
allyl isothiocyanate + glutathione
-
Homo sapiens ?
-
?
benzyl isothiocyanate + glutathione
-
Mus musculus ?
-
?
benzyl isothiocyanate + glutathione
-
Rattus norvegicus ?
-
?
benzyl isothiocyanate + glutathione
-
Homo sapiens ?
-
?
cumene hydroperoxide + glutathione
-
Mus musculus ?
-
?
cumene hydroperoxide + glutathione
-
Rattus norvegicus ?
-
?
cumene hydroperoxide + glutathione
-
Homo sapiens ?
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
-
Mus musculus S-(2,4-dinitrophenyl)glutathione + HCl
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
-
Rattus norvegicus S-(2,4-dinitrophenyl)glutathione + HCl
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
-
Homo sapiens S-(2,4-dinitrophenyl)glutathione + HCl
-
?
glutathione + 1-iodobutane
-
Mus musculus iodide + butyl-glutathione
-
?
glutathione + 1-iodohexane
-
Mus musculus iodide + hexyl-glutathione
-
?
glutathione + 1-iodomethane
-
Mus musculus iodide + methylglutathione
-
?
hydrogen peroxide + glutathione
-
Mus musculus ?
-
?
hydrogen peroxide + glutathione
-
Rattus norvegicus ?
-
?
hydrogen peroxide + glutathione
-
Homo sapiens ?
-
?
iodobutane + glutathione
-
Rattus norvegicus ?
-
?
iodohexane + glutathione
-
Rattus norvegicus ?
-
?
iodomethane + glutathione
-
Rattus norvegicus ?
-
?
additional information substrate specificity, overview. A major structural determinant of substrate recognition is the H-site, which binds the electrophile in proximity to the nucleophilic sulfur of the second substrate glutathione. H-site residue 234 has a key role in governing the activity and substrate selectivity profile of GST T1-1 Mus musculus ?
-
?
additional information substrate specificity, overview. A major structural determinant of substrate recognition is the H-site, which binds the electrophile in proximity to the nucleophilic sulfur of the second substrate glutathione. H-site residue 234 has a key role in governing the activity and substrate selectivity profile of GST T1-1 Rattus norvegicus ?
-
?
additional information substrate specificity, overview. A major structural determinant of substrate recognition is the H-site, which binds the electrophile in proximity to the nucleophilic sulfur of the second substrate glutathione. H-site residue 234 has a key role in governing the activity and substrate selectivity profile of GST T1-1 Homo sapiens ?
-
?
phenethyl isothiocyanate + glutathione
-
Mus musculus ?
-
?
phenethyl isothiocyanate + glutathione
-
Rattus norvegicus ?
-
?
phenethyl isothiocyanate + glutathione
-
Homo sapiens ?
-
?
propyl isothiocyanate + glutathione
-
Mus musculus ?
-
?
propyl isothiocyanate + glutathione
-
Rattus norvegicus ?
-
?
propyl isothiocyanate + glutathione
-
Homo sapiens ?
-
?
styrene oxide + glutathione
-
Mus musculus ?
-
?
styrene oxide + glutathione
-
Rattus norvegicus ?
-
?
styrene oxide + glutathione
-
Homo sapiens ?
-
?
tertiary butyl hydroperoxide + glutathione
-
Mus musculus ?
-
?
tertiary butyl hydroperoxide + glutathione
-
Rattus norvegicus ?
-
?
tertiary butyl hydroperoxide + glutathione
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More the substrate recognition H-site is formed by several segments of amino acid residues located in separate regions of the primary structure. The C-terminal helix of the protein serves as a lid over the active site, and contributes several residues to the H-site, molecular modeling, overview Mus musculus
More the substrate recognition H-site is formed by several segments of amino acid residues located in separate regions of the primary structure. The C-terminal helix of the protein serves as a lid over the active site, and contributes several residues to the H-site, molecular modeling, overview Rattus norvegicus
More the substrate recognition H-site is formed by several segments of amino acid residues located in separate regions of the primary structure. The C-terminal helix of the protein serves as a lid over the active site, and contributes several residues to the H-site, molecular modeling, overview Homo sapiens

Synonyms

Synonyms Comment Organism
GST T1-1
-
Mus musculus
GST T1-1
-
Rattus norvegicus
GST T1-1
-
Homo sapiens
theta class glutathione transferase T1-1
-
Mus musculus
theta class glutathione transferase T1-1
-
Rattus norvegicus
theta class glutathione transferase T1-1
-
Homo sapiens
theta class GST
-
Mus musculus
theta class GST
-
Rattus norvegicus
theta class GST
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mus musculus
30
-
assay at Rattus norvegicus
30
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.037
-
iodobutane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
0.068
-
iodohexane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
0.137
-
1-Iodobutane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
0.215
-
iodobutane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
3.17
-
1-iodohexane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
3.52
-
iodohexane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
9.16
-
iodomethane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
238
-
1-iodomethane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
349
-
iodomethane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8 assay at, dependent on the substrate Mus musculus
6.5 8 assay at, dependent on the substrate Rattus norvegicus
6.5 8 assay at, dependent on the substrate Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.244
-
iodobutane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
0.59
-
iodobutane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
0.701
-
iodobutane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
1.17
-
iodohexane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
54.1
-
iodohexane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
69.8
-
iodohexane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
107
-
iodomethane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
185
-
iodomethane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
263
-
iodomethane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus