Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant isozyme GSTA4-4, and of isozyme GSTA1-1GIMFhelix mutant in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type isozyme GSTA4-4 and recombinant mutant GSTA1-1 GIMFhelix in complex with reaction product 4-hydroxynonenal-3S-glutathione, 0.002 ml of protein solution containing 10 mg/ml protein in 10 mM HEPES, pH 7.0, with a 10fold molar excess of ligand are mixed with 0.002 ml of reservoir solution containing containing 24% PEG 4000, 0.1 M sodium acetate trihydrate, pH 4.6, and 0.2 M ammonium sulfate, or mixing of 0.002 ml protein solution with 500 nl of EtOH and 0.003 ml of reservoir solution containing 16% PEG monomethyl ester 5000, 0.1 M HEPES, pH 7.5, and 10% isopropyl alcohol, X-ray diffraction structure determination and analysis at 1.90-2.10 A resolution, molecular replacement | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant GSTA1-1GIMFhelix | Homo sapiens |
Y9F | site-directed mutagenesis of isozyme GSTA4-4 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
rac-4-hydroxynonenal + glutathione | Homo sapiens | elimination of the lipid peroxidation product 4-hydroxynonenal, a toxic compound that contributes to numerous diseases | S-(4-hydroxy-1-oxononan-3-yl)glutathione | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme GSTA4-4 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant isozyme GSTA4-4, and recombinant isozyme GSTA1-1GIMFhelix mutant from Escherichia coli by glutathione affinity chromatography and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isozyme GSTA4-4 shows high catalytic chemospecificity for alkenals, but negligible stereoselectivity, GSTA4-4 undergoes no enantiospecific induced fit, mechanism of the combination of high substrate chemoselectivity and low substrate stereoselectivity, overview | Homo sapiens | ? | - |
? | |
rac-4-hydroxynonenal + glutathione | elimination of the lipid peroxidation product 4-hydroxynonenal, a toxic compound that contributes to numerous diseases | Homo sapiens | S-(4-hydroxy-1-oxononan-3-yl)glutathione | - |
? | |
rac-4-hydroxynonenal + glutathione | GSTA1-1 isoform has poor catalytic efficiency with 4-hydroxynonenal, GSTA4-4 has negligible stereoselectivity towards the two 4-hydroxynonenal enantiomers, despite its high catalytic chemospecificity for alkenals | Homo sapiens | S-[(2S)-3-hydoxy-2-(2-oxoethyl)octyl]glutathione | product binding, overview | ? |
Synonyms | Comment | Organism |
---|---|---|
glutathione transferase A4-4 | - |
Homo sapiens |
GSTA4-4 | - |
Homo sapiens |