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Literature summary for 2.5.1.18 extracted from

  • Guevercin, S.; Erat, M.; Sakiroqlu, H.
    Determination of some kinetic and characteristic properties of glutathione S-transferase from bovine erythrocytes (2008), Protein Pept. Lett., 15, 6-12.
    View publication on PubMed
Search for more literature for 2.5.1.18

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3257
-
 glutathione
-
 Bos taurus
0.7447
-
 1-chloro-2,4-dinitrobenzene
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 2 * 27000, SDS-PAGE Bos taurus
53000
-
 native enzyme, gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione agarose affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene best substrate Bos taurus S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 27000, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
 Bos taurus
GST
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.95
-
 glutathione
-
 Bos taurus
190.6
-
 1-chloro-2,4-dinitrobenzene
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
 stable at pH 6.5 in potassium phosphate buffer Bos taurus