Literature summary for 2.5.1.18 extracted from

Busenlehner, L.S.; Alander, J.; Jegerscoehld, C.; Holm, P.J.; Bhakat, P.; Hebert, H.; Morgenstern, R.; Armstrong, R.N.
Location of substrate binding sites within the integral membrane protein microsomal glutathione transferase-1 (2007), Biochemistry, 46, 2812-2822.

Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysSL cells	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
E80Q	7% residual activity compared to the wild type enzyme	Rattus norvegicus
H75Q	34% residual activity compared to the wild type enzyme	Rattus norvegicus
N77T	inactive	Rattus norvegicus
R113K	5% residual activity compared to the wild type enzyme	Rattus norvegicus
R63A	30% increased activity compared to the wild type enzyme	Rattus norvegicus
R72A	42% residual activity compared to the wild type enzyme	Rattus norvegicus
R73Q	57% residual activity compared to the wild type enzyme	Rattus norvegicus
S30A	400% increased activity compared to the wild type enzyme	Rattus norvegicus
T64V	280% increased activity compared to the wild type enzyme	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Rattus norvegicus	16020	-
microsome	-	Rattus norvegicus	-	-

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P08011	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.6	-	wild type enzyme, using 1-chloro-2,4-dinitrobenzene as a substrate	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione + 1-chloro-2,4-dinitrobenzene	-	Rattus norvegicus	S-(2,4-dinitrophenyl)glutathione + HCl	-	?

Subunits

Subunits	Comment	Organism
trimer	-	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
MGST1	-	Rattus norvegicus
microsomal glutathione transferase-1	membrane-bound enzyme with both glutathione transferase and hydroperoxidase activities	Rattus norvegicus

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Release 2023.1 (January 2023)