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Literature summary for 2.5.1.18 extracted from
- Cooperative kinetics of the recombinant glutathione transferase of Taenia solium and characterization of the enzyme (2008), Arch. Biochem. Biophys., 477, 372-378.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain JM105 | Taenia solium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
glutathione | recombinant enzyme, at 25°C | Taenia solium |  |
| 1.06 | - |
1-chloro-2,4-dinitrobenzene | recombinant enzyme, at 25°C | Taenia solium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Taenia solium | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
around 26000 Da, SDS-PAGE | Taenia solium |
| 26500 | - |
gel filtration | Taenia solium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Taenia solium | Q3ZJN3 | fragment | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sepharose-glutathione column chromatography | Taenia solium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.22 | - |
using 1,2-dichloro-4-nitrobenzene as a substrate | Taenia solium |
| 0.4 | - |
using trans-2-nonenal as a substrate | Taenia solium |
| 0.53 | - |
using hexa 2,4-dienal as a substrate | Taenia solium |
| 2.48 | - |
using cumene hydroperoxide as a substrate | Taenia solium |
| 3.51 | - |
using ethacrynic acid as a substrate | Taenia solium |
| 51.5 | - |
using 1-chloro-2,4-dinitrobenzene as a substrate | Taenia solium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1,2-dichloro-4-nitrobenzene | - |
Taenia solium | ? | - |
? | |
| glutathione + 1-chloro-2,4-dinitrobenzene | preferred substrate | Taenia solium | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
| glutathione + cumene hydroperoxide | - |
Taenia solium | ? | - |
? | |
| glutathione + ethacrynic acid | - |
Taenia solium | ? | - |
? | |
| glutathione + hexa 2,4-dienal | - |
Taenia solium | ? | - |
? | |
| glutathione + trans-2-nonenal | - |
Taenia solium | ? | - |
? | |
| additional information | no activity with 1,2-epoxy-3-(p-nitrophenoxy)-propane and bromosulfophthalein | Taenia solium | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 26GST | 26.5 kDa isozyme | Taenia solium |
| glutathione S-transferase | - |
Taenia solium |
| GST | - |
Taenia solium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 22.2 | - |
1-chloro-2,4-dinitrobenzene | recombinant enzyme, at 25°C | Taenia solium | |
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