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Literature summary for 2.5.1.18 extracted from
- Purification and biochemical characterization of a novel glutathione S-transferase of the silkworm, Bombyx mori (2008), Afr. J. Biotechnol., 7, 311-316.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| albendazol | 17.87% inhibition at 1 mM | Bombyx mori |  |
| bile acid | 41.78% inhibition at 1 mM | Bombyx mori | |
| NaCl | 26.22% inhibition at 1 mM | Bombyx mori | |
| praziquantel | 31.31% inhibition at 1 mM | Bombyx mori | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
glutathione | at 25°C | Bombyx mori | |
| 0.494 | - |
1-chloro-2,4-dinitrobenzene | at 25°C | Bombyx mori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
SDS-PAGE | Bombyx mori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione agarose column chromatography | Bombyx mori |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.728 | - |
crude extract, at 25°C | Bombyx mori |
| 4610 | - |
after 6332fold purification, at 25°C | Bombyx mori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Bombyx mori | S-(2,4-dinitrophenyl)-glutathione + HCl | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Bombyx mori |
| GST | - |
Bombyx mori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Bombyx mori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 70 | at temperatures ranging from 15°C to 70°C, the enzymatic activity increases with increasing temperature up to 25°C, then the activity starts to decrease | Bombyx mori |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 57.87 | - |
glutathione | at 25°C | Bombyx mori | |
| 65.43 | - |
1-chloro-2,4-dinitrobenzene | at 25°C | Bombyx mori | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
- |
Bombyx mori |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.1 | 9 | at pH values ranging from 5.4-7.1, the enzymatic activity increases with increasing pH, then the activity starts to decrease | Bombyx mori |
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