Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes in Escherichia coli | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type GST in complex with inhibitor S-hexylglutathione, 22°C, hanging drop vapour diffusion method, from 60 mM CaCl2, 30 mM HEPES, pH 7.5, 8.4% PEG 400, 3.4 mg GST/ml, and 1.4 mM S-hexylglutathione, the reservoir solution contains 150 mM CaCl2, 75 mM HEPES, pH 7.5, and 21% PEG 400, X-ray diffraction structure determination and analysis at 2.4 A resolution | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
C101A | site-directed mutagenesis, the mutant shows unaltered ligand binding compared to the wild-type enzyme | Plasmodium falciparum |
C86A | site-directed mutagenesis, the mutant shows unaltered ligand binding compared to the wild-type enzyme | Plasmodium falciparum |
K15E | site-directed mutagenesis, the mutant shows altered ligand binding compared to the wild-type enzyme | Plasmodium falciparum |
Q71E | site-directed mutagenesis, the mutant shows altered ligand binding compared to the wild-type enzyme | Plasmodium falciparum |
Y211F | site-directed mutagenesis, the mutant shows unaltered ligand binding compared to the wild-type enzyme | Plasmodium falciparum |
Y9F | site-directed mutagenesis, the mutant shows unaltered ligand binding compared to the wild-type enzyme | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ferriprotoporphyrin IX | in presence of glutathione, GST serves as ligand for parasitotoxic ferriprotoporphyrin IX with a high- and a low-affinity binding site, uncompetitive inhibition type, overview | Plasmodium falciparum | |
additional information | structural and mechanistic studies on ligand binding and enzyme inhibition, overview | Plasmodium falciparum | |
S-hexylglutathione | binding structure, overview | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
RX + glutathione = HX + R-S-glutathione | substrate binding: Tyr9 is responsible for the deprotonation of GSH and Lys15, but also Gln71 are involved, active site and substrate binding structure, overview | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | substrate binding structure, overview | Plasmodium falciparum | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
additional information | artemisinin is no substrate for glutathione transferase activity but for peroxidase activity, as is cumene hydroperoxide | Plasmodium falciparum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | tertiary and quaternary structure of the enzyme with bound S-hexylglutathione | Plasmodium falciparum |
tetramer | a tetramer that dissociates into dimers in the presence of glutathione | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Plasmodium falciparum |
GST | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Plasmodium falciparum |