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Literature summary for 2.5.1.18 extracted from
- Cloning and characterization of a theta class glutathione transferase from the potato pathogen Phytophthora infestans (2006), Phytochemistry, 67, 1427-1434.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gstt1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Phytophthora infestans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of glutathione peroxidase activity, EC 1.11.1.9, overview | Phytophthora infestans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25452 | - |
2 * 25452, recombinant isozyme GSTT1, mass spectrometry and SDS-PAGE | Phytophthora infestans |
| 46000 | - |
recombinant isozyme GSTT1, gel filtration | Phytophthora infestans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phytophthora infestans | A0AEF0 | theta class isozyme, GSTT1, gene gstt1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant isozyme GSTT1 from Escherichia coli | Phytophthora infestans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | wide tissue distribution of enzyme expression, overview | Phytophthora infestans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the recombinant enzyme shows no activity with 1-chloro-2,4-dinitrobenzene, but is highly active with 9(S)-hydroperoxy-(10E,12Z,15Z)-octadecatrienoic acid, cumene hydroperoxide, and other hydroperoxide substrates as glutathione peroxidase, EC 1.11.1.9, overview | Phytophthora infestans | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 25452, recombinant isozyme GSTT1, mass spectrometry and SDS-PAGE | Phytophthora infestans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GST | - |
Phytophthora infestans |
| More | cf. EC 1.11.1.9 | Phytophthora infestans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57 | - |
10 min, purified recombinant enzyme, stable | Phytophthora infestans |
| 67 | - |
10 min, purified recombinant enzyme, complete inactivation | Phytophthora infestans |
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