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Literature summary for 2.5.1.18 extracted from

  • Scarponi, L.; Quagliarini, E.; Del Buono, D.
    Induction of wheat and maize glutathione S-transferase by some herbicide safeners and their effect on enzyme activity against butachlor and terbuthylazine (2006), Pest Manag. Sci., 62, 927-932.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
benoxacor herbicide safener, activates the enzyme in shoots Triticum aestivum
benoxacor herbicide safener, activates the enzyme in shoots Zea mays
cloquintocet-mexyl herbicide safener, activates the enzyme in shoots Triticum aestivum
cloquintocet-mexyl herbicide safener, activates the enzyme in shoots Zea mays
fenchlorazole-ethyl herbicide safener, activates the enzyme in shoots Triticum aestivum
fenchlorazole-ethyl herbicide safener, activates the enzyme in shoots Zea mays
fenclorim herbicide safener, activates the enzyme in shoots Triticum aestivum
fenclorim herbicide safener, activates the enzyme in shoots Zea mays
fluxofenim herbicide safener, activates the enzyme in shoots Triticum aestivum
fluxofenim herbicide safener, activates the enzyme in shoots Zea mays
additional information no enzyme activation by oxabetrinil, a herbicide safener Zea mays
oxabetrinil herbicide safener, activates the enzyme in shoots Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.557
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of cloquintocet-mexyl Triticum aestivum
0.572
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of fluxofenim Triticum aestivum
0.577
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of fenclorim Triticum aestivum
0.629
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of fenchlorazole-ethyl Triticum aestivum
0.655
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of benoxacor Triticum aestivum
0.749
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C Triticum aestivum
0.753
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of oxabetrinil Triticum aestivum
1.07
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of fluxofenim Zea mays
1.081
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of cloquintocet-mexyl Zea mays
1.342
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of benoxacor Zea mays
1.372
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of fenchlorazole-ethyl Zea mays
1.442
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of fenclorim Zea mays
1.626
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of oxabetrinil Zea mays
1.79
-
1-chloro-2,4-dinitrobenzene pH 6.5, 35°C Zea mays

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
cv. Palesio
-
Zea mays
-
cv. Belgrano
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from shoots by ammonium sulfate fractionation and desalting Triticum aestivum
native enzyme partially from shoots by ammonium sulfate fractionation and desalting Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Triticum aestivum
-
seedling
-
Zea mays
-
shoot
-
Triticum aestivum
-
shoot
-
Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000411
-
substrate butachlor Triticum aestivum
0.00087
-
substrate terbuthylazine Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
Triticum aestivum chloride + 2,4-dinitrophenyl-glutathione
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
-
Zea mays chloride + 2,4-dinitrophenyl-glutathione
-
?
glutathione + butachlor
-
Triticum aestivum ?
-
?
glutathione + butachlor
-
Zea mays ?
-
?
glutathione + terbuthylazine
-
Triticum aestivum ?
-
?
glutathione + terbuthylazine
-
Zea mays ?
-
?

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
Triticum aestivum
glutathione S-transferase
-
Zea mays
GST
-
Triticum aestivum
GST
-
Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Triticum aestivum
35
-
assay at Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Triticum aestivum
6.5
-
assay at Zea mays