Cloned (Comment) | Organism |
---|---|
the catalytically active antibody fragment ScFv2F3 is expressed in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of the catalytically active antibody fragment ScFv2F3 by cloning of VH and VL genes of monoclonal 2F3, elicited against hapten GSH-S-DN2phBu, and sequencing from hybridoma 2F3, followed by protein engineering | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics of the recombinant catalytically active monoclonal antibody fragment ScFv2F3, dependence on substrate concentrations, overview | Mus musculus | |
0.455 | - |
glutathione | pH 6.5, 25°C, recombinant catalytically active monoclonal antibody fragment ScFv2F3 | Mus musculus | |
2.284 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 25°C, recombinant catalytically active monoclonal antibody fragment ScFv2F3 | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
a catalytically active monoclonal antibody fragment | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-bromo-4-nitrobenzene | recombinant catalytically active monoclonal antibody fragment ScFv2F3 | Mus musculus | bromide + 4-nitrophenyl-glutathione | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | recombinant catalytically active monoclonal antibody fragment ScFv2F3 | Mus musculus | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
additional information | the selenomethionine-labeled antibody fragment ScFv2F3 is catalytically inactive | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
44 | - |
recombinant catalytically active monoclonal antibody fragment ScFv2F3 | Mus musculus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | 57.2% of maximal activity at 25°C, 5.6% at 60°C, recombinant catalytically active monoclonal antibody fragment ScFv2F3 | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2286 | - |
glutathione | pH 6.5, 25°C, recombinant catalytically active monoclonal antibody fragment ScFv2F3 | Mus musculus | |
2286 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 25°C, recombinant catalytically active monoclonal antibody fragment ScFv2F3 | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
recombinant catalytically active monoclonal antibody fragment ScFv2F3 | Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | 32% of maximal activity at pH 6.5, recombinant catalytically active monoclonal antibody fragment ScFv2F3 | Mus musculus |