Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.18 extracted from

  • Zhang, K.; Zang, T.Z.; Yang, W.; Sun, Y.; Mu, Y.; Liu, J.Q.; Shen, J.C.; Luo, G.M.
    Single chain antibody displays glutathione S-transferase activity (2006), J. Biol. Chem., 281, 12516-12520.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the catalytically active antibody fragment ScFv2F3 is expressed in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of the catalytically active antibody fragment ScFv2F3 by cloning of VH and VL genes of monoclonal 2F3, elicited against hapten GSH-S-DN2phBu, and sequencing from hybridoma 2F3, followed by protein engineering Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics of the recombinant catalytically active monoclonal antibody fragment ScFv2F3, dependence on substrate concentrations, overview Mus musculus
0.455
-
glutathione pH 6.5, 25°C, recombinant catalytically active monoclonal antibody fragment ScFv2F3 Mus musculus
2.284
-
1-chloro-2,4-dinitrobenzene pH 6.5, 25°C, recombinant catalytically active monoclonal antibody fragment ScFv2F3 Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
a catalytically active monoclonal antibody fragment
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-bromo-4-nitrobenzene recombinant catalytically active monoclonal antibody fragment ScFv2F3 Mus musculus bromide + 4-nitrophenyl-glutathione
-
?
glutathione + 1-chloro-2,4-dinitrobenzene recombinant catalytically active monoclonal antibody fragment ScFv2F3 Mus musculus chloride + 2,4-dinitrophenyl-glutathione
-
?
additional information the selenomethionine-labeled antibody fragment ScFv2F3 is catalytically inactive Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
44
-
recombinant catalytically active monoclonal antibody fragment ScFv2F3 Mus musculus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 57.2% of maximal activity at 25°C, 5.6% at 60°C, recombinant catalytically active monoclonal antibody fragment ScFv2F3 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2286
-
glutathione pH 6.5, 25°C, recombinant catalytically active monoclonal antibody fragment ScFv2F3 Mus musculus
2286
-
1-chloro-2,4-dinitrobenzene pH 6.5, 25°C, recombinant catalytically active monoclonal antibody fragment ScFv2F3 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
recombinant catalytically active monoclonal antibody fragment ScFv2F3 Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 32% of maximal activity at pH 6.5, recombinant catalytically active monoclonal antibody fragment ScFv2F3 Mus musculus