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Literature summary for 2.5.1.18 extracted from
- Characterization of glutathione transferases and glutathione peroxidases in pea (Pisum sativum) (1996), Physiol. Plant., 98, 594-604.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
fluorodifen | - |
Pisum sativum |  |
| 0.55 | - |
glutathione | - |
Pisum sativum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CuCl2 | 0.05 mM and 0.5 mM increases activity of GPOX | Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27500 | - |
1 * 30000 + 1 * 27500 or 1 * 30000 + 1 * 29000, SDS-PAGE, GSTF | Pisum sativum |
| 27500 | - |
1 * 27500 + 1 * 29000, SDS-PAGE, GSTC and GPOX | Pisum sativum |
| 29000 | - |
1 * 30000 + 1 * 27500 or 1 * 30000 + 1 * 29000, SDS-PAGE, GSTF | Pisum sativum |
| 29000 | - |
1 * 27500 + 1 * 29000, SDS-PAGE, GSTC and GPOX | Pisum sativum |
| 30000 | - |
1 * 30000 + 1 * 27500 or 1 * 30000 + 1 * 29000, SDS-PAGE, GSTF | Pisum sativum |
| 48000 | 54000 | gel filtration | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Medicago sativa | - |
alfalfa | - |
| Phaseolus coccineus | - |
runner bean | - |
| Phaseolus vulgaris | - |
french bean | - |
| Pisum sativum | - |
pea | - |
| Trifolium repens | - |
white clover | - |
| Triticum aestivum | - |
- |
- |
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GSTC: activity towards 1-chloro-2,4-dinitrobenzene, GSTF: activity towards the herbicide fluorodifen, GPOX: peroxidase activity | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Triticum aestivum | - |
| root | - |
Pisum sativum | - |
| seedling | etiolated | Pisum sativum | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 30% loss of activity after 16 h | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Phaseolus vulgaris | S-2,4-dinitrophenylglutathione + HCl | - |
? | |
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Triticum aestivum | S-2,4-dinitrophenylglutathione + HCl | - |
? | |
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Pisum sativum | S-2,4-dinitrophenylglutathione + HCl | - |
? | |
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Zea mays | S-2,4-dinitrophenylglutathione + HCl | - |
? | |
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Medicago sativa | S-2,4-dinitrophenylglutathione + HCl | - |
? | |
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Trifolium repens | S-2,4-dinitrophenylglutathione + HCl | - |
? | |
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Phaseolus coccineus | S-2,4-dinitrophenylglutathione + HCl | - |
? | |
| glutathione + cumene hydroperoxide | - |
Pisum sativum | ? | - |
? | |
| glutathione + cumene hydroperoxide | - |
Medicago sativa | ? | - |
? | |
| glutathione + cumene hydroperoxide | - |
Trifolium repens | ? | - |
? | |
| glutathione + fluorodifen | - |
Phaseolus vulgaris | 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione | - |
? | |
| glutathione + fluorodifen | - |
Triticum aestivum | 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione | - |
? | |
| glutathione + fluorodifen | - |
Pisum sativum | 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione | - |
? | |
| glutathione + fluorodifen | - |
Zea mays | 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione | - |
? | |
| glutathione + fluorodifen | - |
Medicago sativa | 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione | - |
? | |
| glutathione + fluorodifen | - |
Trifolium repens | 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione | - |
? | |
| glutathione + fluorodifen | - |
Phaseolus coccineus | 4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione | - |
? | |
| additional information | GSTC: activity towards 1-chloro-2,4-dinitrobenzene, GSTF: activity towards the herbicide fluorodifen, GPOX: peroxidase activity, overview: xenobiotic substrates | Pisum sativum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Triticum aestivum |
| dimer | - |
Zea mays |
| dimer | 1 * 30000 + 1 * 27500 or 1 * 30000 + 1 * 29000, SDS-PAGE, GSTF | Pisum sativum |
| dimer | 1 * 27500 + 1 * 29000, SDS-PAGE, GSTC and GPOX | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.3 | 9.5 | cleavage of fluorodifen | Pisum sativum |
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